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Experimental animal model construction method of removing pineal gland in mice and application thereof

A technology of experimental animals and construction methods, which is applied in the field of animal model construction, can solve the problems of low success rate of model construction, excessive bleeding, and low survival rate of mice, so as to avoid massive intracranial hemorrhage, ensure correctness, and improve surgical efficiency. Effect

Inactive Publication Date: 2017-06-20
JIANGSU INST OF PARASITIC DISEASES
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Problems solved by technology

[0003] The purpose of the present invention is to solve the problems of excessive bleeding in the process of removing the pineal gland of mice in the prior art, the low survival rate of postoperative mice, and the low success rate of model construction, and to provide a method that can quickly and efficiently remove the mouse pineal gland. Experimental Animal Model Construction Method and Application of Pineal Gland

Method used

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  • Experimental animal model construction method of removing pineal gland in mice and application thereof
  • Experimental animal model construction method of removing pineal gland in mice and application thereof
  • Experimental animal model construction method of removing pineal gland in mice and application thereof

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Experimental program
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Effect test

Embodiment 1

[0024] 1. Anesthesia: intraperitoneal injection of pentobarbital sodium 80 mg / kg.

[0025] 2. Hair removal: remove the hair on the top of the mouse's forehead and between the ears.

[0026] 3. Disinfection: Wipe the exposed scalp and its surrounding area with iodophor cotton ball.

[0027] 4. Fixation and incision Ⅰ: Fix the mouse on the operating table, and use a scalpel to make an incision about 10 mm in length from the middle of the two ears of the mouse along the center of the top of the head, from the occipital bone to the parietal bone, and along the sagittal suture direction. Open the scalp, expose the herringbone suture, gently scrape the periosteum on the skull with a scalpel (the mouse will struggle a little during this process, and the mouse head needs to be fixed by hand), the periosteum at the exposed part must be removed, otherwise it will affect Subsequent craniotomy.

[0028] 5. Incision II: directly above the herringbone seam, make an isosceles trapezoid wit...

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Abstract

A method for constructing an experimental animal model for removing the pineal gland of mice and its application, relating to the construction of a mouse experimental animal model for removing the pineal gland, and its application in the field of melatonin research, belonging to the construction of animal models technology field. According to the method, the pineal gland of the mouse is removed under the condition of visibility through surgical operation, so as to construct the mouse experimental animal model of the pineal gland removal. The operation method provided by the present invention is relatively simple, the operation is relatively easy, and the construction cost is low. Compared with the existing pinealectomy, the survival rate of mice and the success rate of model construction are greatly improved, which is an economical method. , convenient and fast method.

Description

technical field [0001] A method for constructing an experimental animal model for removing the pineal gland of a mouse and its application, belonging to the technical field of animal model construction. Background technique [0002] Numerous studies of pineal metabolism have shown that the pineal gland is an excellent research object for investigating biological circadian rhythms. At the same time, the important role played by the pineal gland in the fields of seasonal regulation and light-dependent reproduction has also been widely proved. Melatonin is a hormone mainly secreted by the pineal gland, but about 25% of melatonin is distributed outside the pineal gland. Previous studies have confirmed that melatonin is both a neuroendocrine hormone and an immunomodulator. Existing pinealectomy can easily injure the superior sagittal sinus and transverse sinus, causing fatal hemorrhage. It is difficult to construct an experimental model, and the success rate is low; and the mod...

Claims

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Application Information

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IPC IPC(8): A61D1/00
CPCA61D1/00
Inventor 汪伟
Owner JIANGSU INST OF PARASITIC DISEASES
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