The method of artificially inducing labor of Lhasa schizospigot
A technology of artificial induced labor and naked slits, which is applied in fish farming, pharmaceutical formulations, and medical raw materials derived from fish feed, etc., to achieve strong operability, simple methods, and good practical applicability
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Embodiment 1
[0034] A method for artificially inducing birth of Lhasa naked schizosalis, comprising the following steps:
[0035] A. Parent fish selection
[0036] Select healthy, well-developed, non-obvious trauma and no deformity wild Lhasa nudibranch broodstock, and select good broodstock to be disinfected with potassium permanganate before entering the pool, and then separate the male and female in the broodstock holding pool for one day. ; The temporary breeding pond was soaked with potassium permanganate for one week in advance, and then cleaned with clear water; the water temperature of the broodstock temporary breeding pond was 11°C, the dissolved oxygen was 6mg / L, and the pH was 7.2;
[0037] B. Artificial induced labor
[0038] The pectoral fin base injection method was adopted, and the injection dose was: 5 μg luteinizing hormone releasing hormone analogue / kg+2.5mg carp pituitary gland / kg+1ml fish normal saline;
[0039] Female fish were injected twice, the first injection was...
Embodiment 2
[0045] A method for artificially inducing birth of Lhasa naked schizosalis, comprising the following steps:
[0046] A. Parent fish selection
[0047]Select healthy, well-developed, non-obvious trauma and no deformity wild Lhasa nudibranch broodstock, and select good broodstock to be disinfected with potassium permanganate before entering the pool, and then separate the male and female in the broodstock holding pool for one day. ; The holding pool is soaked with potassium permanganate for one week in advance, and then cleaned with clear water; the water temperature of the broodstock holding pool is 12°C, the dissolved oxygen is 8 mg / L, and the pH is 7.5;
[0048] B. Artificial induced labor
[0049] The pectoral fin base injection method was adopted, and the injection dose was: 5 μg luteinizing hormone releasing hormone analogue / kg+2.5mg carp pituitary gland / kg+1ml fish normal saline;
[0050] Female fish were injected twice, the first injection was 30%, and the second injec...
Embodiment 3
[0056] A method for artificially inducing birth of Lhasa naked schizosalis, comprising the following steps:
[0057] A. Parent fish selection
[0058] Select healthy, well-developed, non-obvious trauma and no deformity wild Lhasa nudibranch broodstock, and select good broodstock to be disinfected with potassium permanganate before entering the pool, and then separate the male and female in the broodstock holding pool for one day. The holding pool was soaked with potassium permanganate for one week in advance, and then cleaned with clean water; the water temperature of the broodstock holding pool was 11.5°C, the dissolved oxygen was 7 mg / L, and the pH was 7.3;
[0059] B. Artificial induced labor
[0060] The pectoral fin base injection method was adopted, and the injection dose was: 5 μg luteinizing hormone releasing hormone analogue / kg+2.5mg carp pituitary gland / kg+1ml fish normal saline;
[0061] Female fish were injected twice, the first injection was 30%, and the second ...
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