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Preparation and application of chiral electrochemical sensor material based on chitosan

A chitosan, electrochemical technology, applied in the direction of material electrochemical variables, material analysis by electromagnetic means, analysis of materials, etc., can solve the problems of poor conductivity of chitosan, limitations of chiral electrochemical sensors, etc.

Inactive Publication Date: 2017-06-27
NORTHWEST NORMAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, due to the poor conductivity of chitosan, it is limited in the construction of chiral electrochemical sensors.

Method used

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  • Preparation and application of chiral electrochemical sensor material based on chitosan
  • Preparation and application of chiral electrochemical sensor material based on chitosan
  • Preparation and application of chiral electrochemical sensor material based on chitosan

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] Embodiment 1, the preparation of PTCA-CS composite material

[0024] (1) Hydrolysis of PTCDA: Weigh 1g of PTCDA, disperse it in 50mL of 1%KOH solution, ultrasonically disperse it for 5min, and then heat it in a water bath for 1h to obtain a solution that is completely soluble in water and uniformly dispersed. , and then gradually add 0.1mol / L HCl solution until 3,4,9,10-perylenetetracarboxylic acid (PTCA) precipitates completely and keeps it slightly acidic; centrifuge the precipitate and wash it several times with twice distilled water to prepare Get PTCA;

[0025] (2) PTCA activation: Weigh 100mg of PTCA, ultrasonic 5min to disperse PTCA evenly in 50mL PBS (pH=7) solution; add 10mg of EDC, stir at room temperature for 0.5h, then add 30mg (NHS), at room temperature Stir continuously for 6 hours to activate its carboxyl group to obtain activated PTCA;

[0026] (3) Protonation of CS: Weigh 100mg CS, add it into 50ml 0.1mol / L acetic acid solution, ultrasonicate for 5min...

Embodiment 2

[0028]Embodiment 2, the construction of electrochemical sensor

[0029] (1) Cleaning of bare glassy carbon electrodes: use 0.3 μm and 0.05 μm Al on the bare glassy carbon electrodes on the suede 2 o 3 Polishing, after washing the surface dirt, transfer to ethanol and ultrapure water and sonicate for 5 minutes respectively;

[0030] (2) Surface modification of glassy carbon electrode: The cleaned bare glassy carbon electrode was placed in 2 mg / mL PTCA-CS aqueous solution, and PTCA-CS was electrodeposited on the electrode surface by cyclic voltammetry (CV) to obtain PTCA-CS CS modified glassy carbon electrode PTCA-CS / GCE. The conditions of cyclic voltammetry are: the scanning potential is from 0 to 1.0V, and the scanning speed is 50mVs -1 , scan 50 circles.

[0031] (3) Identification of tryptophan isomers: The PTCA-CS modified electrodes were placed in 25mL L-tryptophan and D-tryptophan aqueous solutions (concentration: 5mmol / L), and differential pulse voltammetry was used ...

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Abstract

The invention discloses preparation of an electrochemical chiral sensor material based on chitosan and construction of the chiral sensor, and belongs to the fields of composite materials and electrochemical techniques. The preparation comprises the following steps: firstly dispersing PTCA obtained by hydrolyzing PTCDA in an alkali solution into a phosphoric acid buffer salt solution PBS, then adding EDC and NHS to activate carboxyl of PTCA, then adding a protonated chitosan liquid, and preparing the electrochemical chiral sensor material PTCA-CS based on chitosan through an amidation reaction. The PTCA-CS is modified on the surface of a glassy carbon electrode through electrolytic deposition to obtain the chiral electrochemical sensor. Chiral recognition is carried out on tryptophan isomers by using differential pulse voltammetry, so that the chiral electrochemical sensor has greater identification signal for L-tryptophan. Therefore, the L-tryptophan in the tryptophan isomers can be quickly and sensitively identified.

Description

technical field [0001] The present invention relates to an electrochemical chiral sensing and recognition material, in particular to a chitosan-based chiral electrochemical sensor material and its preparation; it is mainly used for the chiral recognition of tryptophan isomers and belongs to Composite material technology field and chiral electrochemical recognition field. Background technique [0002] Chirality is an inherent property of nature and plays an important role in living organs. Microscopic chiral molecules constitute the apparent chirality. In living organisms, the two racemates of chiral substances exist in different amounts, and only one configuration of the living system is developing in a positive direction that is beneficial to life. Living organisms can distinguish biomolecules of different configurations and produce different biomolecules. metabolism. This property of nature makes the biomacromolecules of life activities or their basic constituent units ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08B37/08G01N27/36G01N27/30G01N27/48
CPCC08B37/003G01N27/308G01N27/36G01N27/48
Inventor 莫尊理牛小慧高虎虎李振亮
Owner NORTHWEST NORMAL UNIVERSITY