Chlorophyll fluorescence imaging based transgenic maize glyphosate tolerance phenotype detection method

A technology of transgenic corn and chlorophyll fluorescence, applied in the direction of color/spectral characteristic measurement, fluorescence/phosphorescence, measuring device, etc., can solve the problems of unfavorable popularization and application, labor-intensive, material resources, poor timeliness, etc., and achieves simple, environmentally friendly, and easy-to-operate operations. , avoid the effect of cumbersome process

Active Publication Date: 2017-06-27
ZHEJIANG UNIV
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Problems solved by technology

However, these methods are destructive to samples, consume a lot of manpower and material re

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  • Chlorophyll fluorescence imaging based transgenic maize glyphosate tolerance phenotype detection method
  • Chlorophyll fluorescence imaging based transgenic maize glyphosate tolerance phenotype detection method
  • Chlorophyll fluorescence imaging based transgenic maize glyphosate tolerance phenotype detection method

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Embodiment Construction

[0038] The following is a further detailed description in conjunction with the drawings and embodiments. The specific implementation manners are implemented on the premise of the technical solution of the present invention, and it should be understood that these manners are only used to illustrate the present invention and not to limit the scope of the present invention. Such as figure 1 As shown, the present invention takes the bivalent gene (cry1Ab / cry2Aj-G10evo) transfected corn as an example of implementation, and the phenotype of other plants can be performed with reference to the method of this example.

[0039] 1. Prepare experimental samples. Plant 120 pots of genetically modified (transformed into cry1Ab / cry2Aj-G10evo gene) and non-transgenic maize. When the maize is grown to the 3-leaf stage, the concentration will be 1,080g a.e.ha -1 The glyphosate solution was placed in a portable CO2 high-pressure sprayer, and 80 transgenic and non-transgenic corn plants were sprayed...

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Abstract

The invention discloses a chlorophyll fluorescence imaging based transgenic maize glyphosate tolerance phenotype detection method. The method includes: (1) preparing experimental samples; (2) acquiring shikimic acid contents of canopy leaves of transgenic maize and non-transgenic maize plants subjected to glyphosate stress in different day numbers; (3) acquiring canopy chlorophyll fluorescence parameters of the transgenic maize and the non-transgenic maize plants subjected to glyphosate stress in different day numbers through a chlorophyll fluorescence imaging system; (4) establishing a PLSR model to reject abnormal samples, and selecting a modeling set and a prediction set through K-Means; (5) selecting the chlorophyll fluorescence parameters most sensitive in glyphosate stress response by means of principal component analysis; (6) establishing a PLSR regression analysis model on the basis of the selected chlorophyll fluorescence parameters and the practical shikimic acid content of the transgenic maize plants, wherein determination coefficients R<2> of the modeling set and the prediction set are 0.75 and 0.63 respectively. The method is high in identification precision and simple in operation, and quick nondestructive testing of transgenic maize glyphosate tolerance phenotypes can be realized.

Description

Technical field [0001] The invention belongs to the technical field of non-destructive detection of herbicide tolerance of genetically modified crops, and specifically relates to a method for detecting the glyphosate tolerance phenotype of genetically modified corn based on chlorophyll fluorescence imaging. Background technique [0002] With the rapid development of modern agriculture, farmland weeds that affect the development of agricultural production have received widespread attention. Studies have shown that field weeds affect the quality of crops, and can also cause crop yields ranging from 10% to 100% loss, seriously threatening agricultural production. At present, the control of weeds mainly relies on spraying herbicides. Glyphosate is the most successful herbicide developed in the 1970s. Because of its broad weed-killing spectrum and excellent systemic conductivity, it can effectively control perennial deep-rooted malignant weeds, is safe for humans and animals, is eas...

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Application Information

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IPC IPC(8): G01N21/33G01N21/64
CPCG01N21/33G01N21/6486
Inventor 冯旭萍何勇刘小丹
Owner ZHEJIANG UNIV
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