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Method of quickly purifying protease of viscera of sleeve-fish and application

A technology of squid viscera and protease, which is applied in the direction of biochemical equipment and methods, enzymes, hydrolytic enzymes, etc., can solve the problems that the purity and enzyme activity of protease preparations need to be improved, and achieve the goal of increasing the added value of fishery production, high purity, and inhibiting bacteria breeding effect

Inactive Publication Date: 2017-06-30
ZHEJIANG OCEAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The purity and enzyme activity of the protease preparation prepared by this method all need to be improved

Method used

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  • Method of quickly purifying protease of viscera of sleeve-fish and application

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Effect test

Embodiment 1

[0018] A method for rapidly purifying squid viscera protease, the specific operation steps:

[0019] 1) Pretreatment: Remove squid ink sacs, squid skin, and squid eyes from squid viscera, keep liver, pancreas, and intestines, wash squid viscera with detergent for 1 to 2 times, and then wash with water for 1 to 4 times. The ingredients of the cleaning agent and their parts by weight are: 3~6 parts of chitin, 5~10 parts of activated carbon, 1~2 parts of mugwort leaf extract, 0.0003~0.0008 parts of magnesium aspartate and 2~5 parts of oyster shells calcined at high temperature pink. In addition to effectively removing the oil stains on the surface of squid viscera, the above-mentioned cleaning agent can also remove bacteria and inhibit the growth of bacteria;

[0020] 2) Preparation of crude enzyme solution: Cut squid viscera into small pieces with clean and rust-free scissors, add deionized water, and the volume ratio of squid viscera fragments: deionized water is 1:1.5~3.0. U...

Embodiment 2

[0026] A kind of rapid purification squid viscera protease method, the most preferred operation steps:

[0027] 1) Pretreatment: Remove squid ink sacs, squid skin, and squid eyes from squid viscera, keep liver, pancreas, and intestines, wash the squid viscera once with a cleaning agent, and then wash them 3 times with water. The ingredients of the cleaning agent and the most preferred parts by weight are: 4 parts of chitin, 8 parts of activated carbon, 2 parts of mugwort leaf extract, 0.0006 parts of magnesium aspartate and 4 parts of oyster shell powder calcined at high temperature. In addition to effectively removing the oil stains on the surface of squid viscera, the above-mentioned cleaning agent can also remove bacteria and inhibit the growth of bacteria;

[0028] 2) Preparation of crude enzyme solution: Cut squid viscera into small pieces with clean and rust-free scissors, add deionized water, and the volume ratio of squid viscera fragments: deionized water is 1:2. Squi...

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Abstract

The invention provides a method of quickly purifying protease of viscera of sleeve-fish. The method specifically comprises the following steps of: 1) pre-treatment; 2) preparation of a crude enzyme liquid; (3) purification by an IPS method; 4) membrane separation and purification; and 5) drying. The method provided by the invention has the beneficial effects that the protease of viscera of the sleeve-fish can be quickly purified; compared with the crude enzyme liquid, the protease of viscera of the sleeve-fish is purified by 3.68 times, the specific enzyme activity reaches 8.21 U / mg, and the protein recovery rate is 63.64% through ISP treatment; and through ultra-filtration, the protease is purified by 3.96 times, the specific enzyme activity reaches 8.83 U / mg, and the protein recovery rate is 54.55%.

Description

technical field [0001] The invention relates to the technical field of protease purification, in particular to a method for rapidly purifying squid viscera protease. Background technique [0002] The method used to prepare the self-solution of squid viscera in the early stage is the autolysis method. The autolysis method is based on making full use of one or more enzymes contained in the squid viscera itself. Therefore, further understanding of squid viscera protease will help the development and utilization of squid viscera resources. At present, many enzymes have been isolated from aquatic products or their leftovers, such as trypsin, chymotrypsin, pepsin, cathepsin B, cathepsin D, and cathepsin E, etc. However, little is known about the protease species and distribution in the viscera of North Pacific squid. [0003] In general, common purification methods for proteases include salting out, extraction, chromatography, and two-phase or three-phase separation. Rational u...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N9/64
CPCC12N9/6405
Inventor 宋茹韦荣编张开强
Owner ZHEJIANG OCEAN UNIV
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