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A straw decomposing agent and its preparation method and application
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A straw decomposing agent and seed technology, which is applied in the field of straw decomposing agent, can solve the problems that farmers are not easy to accept, the operation is cumbersome, and it cannot play the role of promoting decomposition.
Active Publication Date: 2021-01-29
德强生物股份有限公司
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Problems solved by technology
Although the company's research and development center has made some progress in the research of the straw decomposing agent straw decomposing agent of the present invention, it is a must to increase the type and quantity of microorganisms in the process of applying the straw decomposing agent, and finally improve the comprehensive effect and efficiency of decomposition. This long-term and arduous experimental process requires a lot of manpower and material resources to complete. At present, there are some problems and limitations in the use of straw decomposing agents.
For example: 1. It is limited by climatic conditions such as temperature and humidity, especially in the Northeast region. After the straw is returned to the field in October, the temperature and humidity will drop. Most straw decomposing agents cannot be used without suitable temperature and humidity. play a decomposing role
2. The method of using the straw decomposing agent is too cumbersome, and it may not be easy for farmers to accept it in practical applications.
3. At present, the cost of straw decomposing agent sold in the market is relatively high.
Method used
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preparation example Construction
[0069] The preparation of embodiment 1 Trichoderma aculeatus fermented bacterial liquid
[0070] (1) Preparation of primary fermented seeds
[0071] Inoculate Trichoderma asperellum DQ-T36 (CCTCC NO: M 2016449) in shake flask culture medium, culture at 28°C and 240r / min for 24 hours, then check under microscope to confirm the hyphae Strong growth, no bacteria, as a primary fermentation seed;
[0072] Wherein, every 1L of the medium contains KH 2 PO 4 2.0g, (NH 4 ) 2 SO 4 1.0g, MgSO 4 ·7H 2 O1.0g, glucose 20.0g, yeast extract powder 1.0g, peptone 10.0g, solvent is deionized water, pН7.2;
[0074]The primary fermented seeds prepared in step (1) are inoculated into the medium in a fully automatic fermenter with a total volume of 5 liters according to the inoculum size of 5% (v / v). The initial pH value is 7.2 and the temperature is After cultivating for 9 hours at 28°C, with a rotation speed of 200r / min and an aeratio...
Embodiment 2
[0076] The preparation of embodiment 2 straw decomposing agent (liquid preparation)
[0077] Add fermented liquor weight 0.2% potassium sorbate, 5% chitosan, and 3% bamboo vinegar to the fermented liquor fermented in Example 1, and the mixture was stirred at a speed of 250r / min for 20 minutes to obtain the Straw decomposing agent, in which the number of viable spores of Trichoderma aculeatus is not less than 10 8 CFU / ml.
Embodiment 3
[0078] The preparation of embodiment 3 straw decomposing agent (liquid preparation)
[0079] Add the potassium sorbate of fermented liquid weight 5% in the fermented liquid fermented in embodiment 1, the chitosan of 2%, the bamboo vinegar of 5.0%, the mixture is stirred at the speed of 300r / min for 10 minutes, obtains the described Straw decomposing agent, in which the number of viable spores of Trichoderma aculeatus is not less than 10 8 CFU / ml.
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Abstract
The invention discloses a straw decomposing agent, a preparation method and application thereof. The main active ingredient in the straw decomposing agent of the present invention is Trichoderma asperellum (Trichoderma asperellum), named Trichoderma asperellum DQ‑T36, which is preserved in China Center for Type Culture Collection, and its strain preservation number is CCTCC NO: M 2016449. The straw decomposing agent of the present invention can be prepared into two kinds of preparations: liquid preparation and solid preparation. The present invention determines the straw decomposing ability, soil physical and chemical properties, nutrient influence, acid fiber, neutral fiber, lignocellulose and other factors in the straw after applying the straw decomposing agent to the wheat straw and corn straw. The effect and advantages of the product provide a theoretical basis for the application effect and application method of the straw decomposing agent, and also provide an effective way for the biodegradation of straw.
Description
technical field [0001] The invention relates to a straw decomposing agent, in particular to a straw decomposing agent which uses Trichoderma asperellum as an active ingredient, and belongs to the technical field of agricultural production. Background technique [0002] As a large agricultural country, according to relevant statistics, our country can produce more than 700 million tons of straw every year. The burning of a large amount of straw not only brings strong pollution to the atmosphere, but also makes us feel very uncomfortable in the living environment. Smoke contains a large amount of toxic and harmful gases such as carbon monoxide, carbon dioxide and sulfur dioxide, which endanger our bodies. A large number of straw burning also exacerbated the greenhouse effect, making the temperature continue to rise. At the same time, burning straw is a waste of resources. Burning straw will reduce the original humus content in the soil, resulting in soil compaction and reduce...
Claims
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Application Information
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