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Acinetobacter baumannii selective enrichment solution and preparation method thereof

A selective technology for Acinetobacter baumannii, applied in the field of microbiological inspection, can solve the problems of selective enrichment solution that have not yet been seen

Active Publication Date: 2020-09-04
SHANGHAI JIKONG BIOTECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

For the promotion and application of Acinetobacter baumannii, the key lies in the development of suitable enrichment solution and culture substrate. At present, the classic solid medium used for enrichment of Acinetobacter baumannii is mainly conventional solid medium. Invention patent for selective enrichment liquid of Anitobacter

Method used

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  • Acinetobacter baumannii selective enrichment solution and preparation method thereof
  • Acinetobacter baumannii selective enrichment solution and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] Acinetobacter baumannii selective enrichment liquid and preparation method thereof are specifically:

[0024] (1) Weighing of basic powder: weigh 10g of tryptone, 10g of soytone, 5g of yeast powder, and 5g of sodium chloride, and place them in a clean and dry container;

[0025] (2) Mixing of raw materials: add 1000mL of purified water to the container in step (1), and stir the mixture in the container with a glass rod to make it uniform;

[0026] (3) Autoclaving: The mixture in step (2) is subjected to high-pressure steam sterilization, the temperature of damp heat sterilization is 121° C., the sterilization time is 15 minutes, and it is cooled to 50° C. after sterilization;

[0027] (4) Supplementary additives: add 10 mg of vancomycin, 25 mg of polymyxin, and 50 mg of cycloheximide to the mixed solution in step (3), pay attention to aseptic operation during this process;

[0028] (5) Subpackage: Subpackage is carried out under aseptic conditions, and the subpackage v...

Embodiment 2

[0030] Acinetobacter baumannii selective enrichment liquid and preparation method thereof are specifically:

[0031] (1) Weighing of basic powder: weigh 50g of tryptone, 0.2g of soytone, 18g of yeast powder, and 6g of sodium chloride, and place them in a clean and dry container;

[0032] (2) Mixing of raw materials: add 1000mL of purified water to the container in step (1), and stir the mixture in the container with a glass rod to make it uniform;

[0033] (3) Autoclaving: The mixture in step (2) is subjected to high-pressure steam sterilization, the temperature of damp heat sterilization is 115° C., the sterilization time is 20 minutes, and the mixture is cooled to 50° C. after sterilization;

[0034] (4) Supplementary additives: add 15 mg of vancomycin, 30 mg of polymyxin, and 75 mg of cycloheximide to the mixed solution in step (3), and pay attention to aseptic operation during this process;

[0035] (5) Subpackage: Subpackage is carried out under sterile conditions, and t...

Embodiment 3

[0037] Acinetobacter baumannii selective enrichment liquid and preparation method thereof are specifically:

[0038] (1) Weighing of basic powder: weigh 15g of tryptone, 10g of soytone, 3g of yeast powder, and 10g of sodium chloride, and place them in a clean and dry container;

[0039] (2) Mixing of raw materials: add 1000mL of purified water to the container in step (1), and stir the mixture in the container with a glass rod to make it uniform;

[0040] (3) Autoclaving: The mixture in step (2) is subjected to high-pressure steam sterilization, the temperature of damp heat sterilization is 121° C., the sterilization time is 20 minutes, and the mixture is cooled to 50° C. after sterilization;

[0041] (4) Supplementary additives: add 10 mg of vancomycin, 3 mg of polymyxin, and 15 mg of cycloheximide to the mixed solution in step (3), pay attention to aseptic operation during this process;

[0042] (5) Subpackage: Subpackage is carried out under sterile conditions, and the sub...

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Abstract

The present invention relates to a selective Acinetobacter baumannii increasing liquid, which comprises 0.1-5% of tryptone, 0.02-1.5% of soybean peptone, 0.03-1.8% of yeast powder, 0.05-1.5% of sodium chloride, 0.1*10<-4>-1.5*10<-3>% of vancomycin, 0.2*10<-4>-3*10<-3>% of polymyxin, and 3*10<-4>-7.5*10<-3>% of actidione according to a mass volume ratio of. The invention further provides a preparation method of the selective Acinetobacter baumannii increasing liquid. According to the present invention, with the application of the selective Acinetobacter baumannii increasing liquid, Acinetobacter baumannii can be selectively culture, the growth of of Gram-positive bacteria can be effectively inhibited, and Escherichia coli, Klebsiella pneumoniae, Pseudomonas aeruginosa, Salmonella, Shigella and other Enterobacter, and other Gram-negative bacteria and fungi.

Description

technical field [0001] The invention relates to the technical field of microbial inspection, in particular to the inspection of Acinetobacter baumannii, in particular to a selective enrichment liquid for Acinetobacter baumannii and a preparation method thereof. Background technique [0002] Acinetobacter baumannii is a Gram-negative bacillus that does not ferment sugars, is oxidase-negative, and cannot move. It is widely distributed in nature, hospital environments, human body surfaces, and upper respiratory tracts. important opportunistic pathogens. The cell size is 1-1.5×1.5-2 microns, without spores and flagella, aerobic, and the optimum temperature is 37°C, but it can also grow at 41-44°C. [0003] In recent years, infections caused by Acinetobacter baumannii have increased significantly. Among non-fermenting bacterial infections, its clinical positive isolation rate is second only to Pseudomonas aeruginosa. drug resistance, strong drug resistance, and serious multidru...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C12R1/01
CPCC12N1/20
Inventor 居建华杨艳杨子江
Owner SHANGHAI JIKONG BIOTECH