Method for doubling eggplant haploid plants

A haploid, eggplant technology, applied in the fields of botanical equipment and methods, plant genetic improvement, application, etc., can solve problems such as poor results

Inactive Publication Date: 2017-07-21
JINLING INST OF TECH
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0005] The technical problem to be solved by the present invention is to provide a method for

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  • Method for doubling eggplant haploid plants
  • Method for doubling eggplant haploid plants
  • Method for doubling eggplant haploid plants

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Embodiment 1

[0030] The experiment started on August 8, 2016, in the Genetics and Breeding Laboratory of Jinling Institute of Science and Technology. The haploid plants are numbered 84-2 and 234, which are eggplant haploid plants obtained from anther culture in 2015 and 2016, respectively, among which 84-2 is a plant preserved from cuttings. 50%wp carbendazim powder is produced by Shanghai Yuelian Chemical Co., Ltd., and its active ingredient content is 50wt%, and every liter of tap water contains 1.25 grams of powder, which is configured as a suspension diluted 800 times; colchicine powder (molecular weight is 399.49) is the original packaging of sigma company, colchicine powder is directly dissolved with ultrapure water, and is configured into solutions of two concentrations of 0.05wt% and 0.2wt%; indole-3-butyric acid (IBA, molecular weight is 203.24) , produced by China Pharmaceutical Group Shanghai Chemical Reagent Company, the powder is first dissolved with a small amount of 100% alc...

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Abstract

The invention discloses a method for doubling eggplant haploid plants. The method includes operation of branch clipping, colchicine solution soaking, doubling treatment, branch pruning, branch cutting, post-cutting heat preservation and moisture preservation management and the like. Compared with the prior art, the method has the advantages that stored haploid plants can be successfully doubled, doubled plants which survive after cutting can be quickly in reproductive growth stages, flower and bear fruits, and selfed seeds can be obtained; cutting and doubling are combined with each other, and accordingly the eggplant haploid plants can be effectively stored, studied and utilized; the survival rates of branches can reach 10% at least in seriously polluted seasons in summers, and the doubling rates of survived plants can range from 50% to 100%.

Description

technical field [0001] The invention belongs to the field of plant breeding or tissue culture, and in particular relates to a method for doubling eggplant haploid plants. Background technique [0002] Eggplant (Solarium melongena L), native to tropical Asia, is cultivated in various provinces of my country. Herb or subshrub, the fruit can be used as vegetable food, and the roots, stems, leaves and seeds can be used as medicine. [0003] The biotechnology developed in recent years, dominated by tissue culture and genetic engineering, has opened up a new way to shorten the breeding period. In the early 1970s, Riana et al. successfully cultivated eggplant anthers and formed seedlings through the callus approach. At present, there have been many successful reports on the cultivation of eggplant anthers or pollen (microspores). Parents, there are not many reports on the breeding of new varieties of eggplant. The reason is mainly that the frequency of eggplant anther or pollen ...

Claims

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Application Information

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IPC IPC(8): A01H1/08
CPCA01H1/08
Inventor 崔群香刘卫东宰学明朱士农
Owner JINLING INST OF TECH
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