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Mixed growth-promoting bacteria for strengthening Artemisia perenniali to remediate cadmium-contaminated soil and its preparation method and application

A technology of cadmium-contaminated soil and growth-promoting bacteria, which is applied in the field of soil treatment to achieve the effect of improving rhizosphere micro-ecology and improving defense capabilities

Active Publication Date: 2020-12-18
HUNAN CITY UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

In recent years, some progress has been made in the research of plant-microbe joint remediation of heavy metal-contaminated soils. However, the optimized combination of growth-promoting strains with heavy metal resistance and belonging to different species is inoculated to plants, which is not effective for remediation plants, especially for wetland remediation plants. There are few reports on the impact of remediation of heavy metal-contaminated soil

Method used

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  • Mixed growth-promoting bacteria for strengthening Artemisia perenniali to remediate cadmium-contaminated soil and its preparation method and application
  • Mixed growth-promoting bacteria for strengthening Artemisia perenniali to remediate cadmium-contaminated soil and its preparation method and application
  • Mixed growth-promoting bacteria for strengthening Artemisia perenniali to remediate cadmium-contaminated soil and its preparation method and application

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preparation example Construction

[0023] The present invention also provides a preparation method of mixed growth-promoting bacteria for strengthening Artemisia perenniali to restore cadmium-contaminated soil, comprising the following steps:

[0024] Step 1. Prepare Bacillus sp.T3 bacterial suspension: On the clean workbench, pick a small amount of T3 bacterial cells with a sterile inoculation loop and inoculate them in a triangular flask containing liquid LB medium, in a shaking incubator, 25- Shake culture at 35°C, 120-180rpm for 12-20h, centrifuge the bacterial solution at 3-5°C, 5000-10000rpm for 4-6min, rinse twice with sterile normal saline, and adjust the concentration of the bacterial suspension to 6.0 with sterile normal saline ×10 10 cfu / mL-7.6×10 10 cfu / mL, prepare Bacillus sp.T3 bacterial suspension;

[0025] Step 2. Prepare Pseudomonasfluorescens Y5 bacterial suspension: On the clean workbench, pick a small amount of Y5 bacterial cells with a sterile inoculation loop and inoculate them into a tr...

Embodiment 1

[0031] A method for preparing mixed growth-promoting bacteria for strengthening Artemisia perenniali to repair cadmium-contaminated soil, comprising the following steps:

[0032] Step 1. Prepare Bacillus sp.T3 bacterial suspension: On the clean workbench, pick a small amount of T3 bacterial cells with a sterile inoculation loop and inoculate them into a triangular flask containing liquid LB medium, place in a shaking incubator at 30°C , 150rpm shaking culture for 16h, the bacterial solution was centrifuged at 4°C and 8000rpm for 5min, rinsed twice with sterile normal saline, and the concentration of the bacterial suspension was adjusted to 6.8×10 10 cfu / mL, prepare Bacillus sp.T3 bacterial suspension;

[0033]Step 2. Prepare Pseudomonasfluorescens Y5 bacterial suspension: On the clean workbench, use a sterile inoculation loop to pick a small amount of Y5 bacterial cells and inoculate them into a conical flask containing liquid LB medium, place in a shaking incubator at 30°C, ...

Embodiment 2

[0039] A method for preparing mixed growth-promoting bacteria for strengthening Artemisia perenniali to repair cadmium-contaminated soil, comprising the following steps:

[0040] Step 1. Prepare Bacillus sp.T3 bacterial suspension: On the clean workbench, pick a small amount of T3 bacterial cells with a sterile inoculation loop and inoculate them into a triangular flask containing liquid LB medium, place in a shaking incubator at 25°C , 180rpm shaking culture for 12h, the bacterial solution was centrifuged at 5°C and 5000rpm for 6min, rinsed twice with sterile normal saline, and adjusted the concentration of the bacterial suspension to 6.0×10 10 cfu / mL, prepare Bacillus sp.T3 bacterial suspension;

[0041] Step 2. Prepare Pseudomonasfluorescens Y5 bacterial suspension: On the clean workbench, pick a small amount of Y5 bacterial cells with a sterile inoculation loop and inoculate them in a triangular flask containing liquid LB medium, in a shaking incubator at 25°C, 180rpm Sha...

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Abstract

The invention discloses a mixed growth-promoting bacterium for strengthening Artemisia perenniali to repair cadmium-contaminated soil and its preparation method and application. The mixed growth-promoting bacterium includes Bacillus sp. solution and Pseudomonas fluorescens Y5 bacterial suspension, wherein the concentration of Bacillus sp.T3 bacterial suspension is 6.0×10 10 cfu / mL-7.6×10 10 cfu / mL; the concentration of Pseudomonas fluorescens Y5 bacterial suspension is 4.8×10 9 cfu / mL‑6.2×10 9 cfu / mL. The mixed growth-promoting bacterium of the invention can improve the rhizosphere micro-ecology of Artemisia annua and can improve the defense ability of Artemisia annua.

Description

technical field [0001] The application belongs to the field of soil treatment, and specifically relates to a mixed growth-promoting bacterium for strengthening Artemisia perenniali to restore cadmium-contaminated soil, its preparation method and application. Background technique [0002] Dongting Lake Wetland is a famous wetland with an important reputation in my country and even in the world, and it is also an important agricultural production base in Hunan Province. Due to factors such as unreasonable mining of mineral resources and waste discharge, Dongting Lake wetland soil has a high content of heavy metals. Studies have shown that the content of heavy metal cadmium in Dongting Lake wetland soil exceeds the level of the national third-class soil. Cadmium is "the first of the five poisons (cadmium, mercury, lead, chromium, arsenic)" and has carcinogenic and teratogenic effects. Long-term accumulation in the soil not only affects the ecological function of the soil, but a...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20B09C1/10C12R1/07C12R1/39
CPCB09C1/10B09C1/105C12N1/20
Inventor 周小梅董萌张擎
Owner HUNAN CITY UNIV
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