Method for detecting influence of electronic cigarette products on relative cell proliferation ratio

Abstract

The invention discloses a method for detecting influence of electronic cigarette products on relative cell proliferation ratio. The method comprises the steps of tested object pretreatment, preparation of a human lung fibroblast single-cell suspension, human lung fibroblast cell concentration calculation, human lung fibroblast cell inoculation, tested object grouping, tested object incubation, tested object incubation product dyeing, tested object incubation product light absorption value measurement, tested product incubation product inhibition ratio calculation, tested object incubation product relative cell proliferation ratio calculation and the like. The method for detecting the influence of the electronic cigarette products on the relative cell proliferation ratio achieves comprehensive survey on the applied characteristics and action manners of the electronic cigarette products, establishes an electronic cigarette product sample pretreatment method, and according to the action target cells of the electronic cigarette products, selects human lung fibroblast cells as electronic cigarette product toxicity testing and detecting cells and determines sample detecting dosage, thereby forming a cell toxicity testing and detecting method applicable to the electronic cigarette products.

Description

technical field [0001] The invention belongs to the application field of biotechnology, and particularly relates to a method for detecting the influence of electronic cigarette products on the relative proliferation rate of cells. Background technique [0002] The relative growth rate (RGR) of cells is a key indicator of cytotoxicity evaluation, and it is often used in the classification of cytotoxicity in the development of new drugs and the safety evaluation of medical devices. The neutral red method and the thiazolium blue method (MTT) are simple, fast, and sensitive, and they are currently two widely used toxicological detection methods for cytotoxicity. In 2002, CORESTA, the International Tobacco Scientific Research Cooperation Center, established a working group for in vitro toxicological testing of cigarette smoke. Potential cytotoxicity is detected, and this detection method has been widely adopted by domestic and foreign tobacco companies. [0003] The increasingl...

AI Technical Summary

Problems solved by technology

No smoke is produced during use, and its use method is significantly different from traditional cigarettes. Changes in the test object lead to differences in sample pretreatment methods, test cells, and test doses. It is used to detect neutrophil toxicity of traditional cigarette cytotoxicity The method cannot meet the needs of genotoxicity testing of e-cigarette products, and at present domestic and foreign research institutions and tobacco companies have not yet formulated a standard method for cytotoxicity testing of e-cigarette products

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