Preparation method of cellulosic ethanol

A technology of cellulosic ethanol and cellulose, applied in the field of preparation of cellulosic ethanol, can solve the problems of incomparability and high cost of cellulosic ethanol, and achieve the effects of reducing preparation cost, reducing control difficulty and reducing load

Inactive Publication Date: 2017-07-21
广西轻工业科学技术研究院有限公司 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there are still many key technical bottlenecks in this field that have not been well resolved, resulting in high cost of cellulosic ethanol, which cannot be compared with starchy raw material ethanol

Method used

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  • Preparation method of cellulosic ethanol

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Experimental program
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Effect test

Embodiment 1

[0040] Crush the banana stalks into 5mm lengths and mix them evenly with the acid solution. With natural pH, blast at 190°C for 10 minutes for acidification blasting, so as to obtain materials that are initially degraded and suitable for biomass conversion. Mix 65°Bx with materials that are initially degraded and suitable for biomass conversion and adjust the pH to 3.5, then add enzymes and mix evenly, and enzymatically hydrolyze at 60°C for 50 hours to obtain mash. Adjust the pH of the mash to 4, add yeast that can use xylose to ferment ethanol for fermentation, ferment until the 10th hour, add Trichoderma reesei, ferment until the 16th hour, add Saccharomyces cerevisiae, and continue to ferment for 48 hours, A cellulosic ethanol-containing feed is obtained from which ethanol is isolated. The amount of yeast, Trichoderma reesei, and Saccharomyces cerevisiae that can ferment ethanol from xylose is 5% of the substrate mass. The results are shown in Table 1.

Embodiment 2

[0042] Crush the banana stalks into 10mm lengths and mix them evenly with the acid solution. With a natural pH, blast at 210°C for 7 minutes for acid blasting, so as to obtain materials that are initially degraded and suitable for biomass conversion. Mix 42°Bx with materials that are initially degraded and suitable for biomass conversion and adjust the pH to 4.0, then add enzymes and mix evenly, and enzymatically hydrolyze at 47°C for 67 hours to obtain mash. Adjust the pH of the mash to 4.5, add yeast that can use xylose to ferment ethanol for fermentation, and ferment until the 6th hour, add Trichoderma reesei, ferment until the 12th hour, add Saccharomyces cerevisiae, and continue to ferment for 40 hours, A cellulosic ethanol-containing feed is obtained from which ethanol is isolated. The amount of yeast, Trichoderma reesei and Saccharomyces cerevisiae that can ferment ethanol from xylose is 10% of the substrate mass. The results are shown in Table 1.

Embodiment 3

[0044] Crush the banana stalks into 25mm lengths and mix them evenly with the acid solution. With natural pH, blast at 250°C for 3 minutes for acidification blasting, so as to obtain materials that are initially degraded and suitable for biomass conversion. Mix the 35°Bx with the materials that are initially degraded and suitable for biomass conversion and adjust the pH to 4.5, then add enzymes and mix evenly, and enzymatically hydrolyze at 40°C for 90 hours to obtain mash. Adjust the pH of the mash to 5, add yeast that can use xylose to ferment ethanol for fermentation, and ferment until the 6th hour, add Trichoderma reesei, ferment to the 10th hour, add Saccharomyces cerevisiae, and continue to ferment for 30 hours, A cellulosic ethanol-containing feed is obtained from which ethanol is isolated. The amount of yeast, Trichoderma reesei and Saccharomyces cerevisiae that can ferment ethanol from xylose is 12% of the substrate mass. The results are shown in Table 1.

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Abstract

The invention discloses a preparation method of cellulosic ethanol. The method includes the steps of firstly, performing explosion pretreatment on a cellulose raw material to obtain a preliminarily degraded material suitable for biomass conversion; secondly, mixing molasses with the material obtained in the first step, regulating pH to 3.5-4.5, and performing enzymolysis to obtain fermenting mash; regulating the pH of the fermenting mash obtained in the second step to 4-5, adding yeast which can use xylose fermentation to obtain ethanol to perform fermentation, adding Trichoderma reesei when the fermentation is performed for 6-10 hours, adding saccharomyces cerevisiae when the fermentation is performed for 10-16 hours, continuing fermenting for 30-48 hours to obtain a material containing the cellulosic ethanol, and separating ethanol from the material. The preparation method has the advantages that the ethanol is prepared by the effective resource utilization of the cellulose and the molasses, pentose utilization degree is increased, raw material use amount is lowered, and production cost and environment pollution can be lowered evidently.

Description

technical field [0001] The invention relates to the fields of biochemical industry, fermentation engineering and biomass energy, in particular to a preparation method of cellulose ethanol. Background technique [0002] With the increasingly prominent energy crisis and environmental pollution, the world's energy development is entering a new period, that is, the world's energy structure is undergoing a change from fossil energy to renewable energy. Cellulosic ethanol has become a research hotspot in industrial biotechnology because it is regarded as the best liquid alternative fuel and has ecological benefits. [0003] In China, the main raw materials for fuel ethanol are crops such as corn and wheat. With the rapid growth of fuel ethanol, the problem of raw materials has become increasingly prominent, which has become a bottleneck restricting the growth of fuel ethanol; in addition, the fuel ethanol industry that uses food crops as raw materials may also cause national food...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P7/10
CPCC12P7/10C12P2201/00Y02E50/10
Inventor 卢国伟谢晓航卓义敏盘柳萍蒋敬全韩宏明张家伟覃红梅严明奕罗虎孙振江李永恒李志鹏许旺发梁坤国鲁佰成宋金凤
Owner 广西轻工业科学技术研究院有限公司
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