Method for detecting feline herpes virus
A feline herpes virus and purpose technology, applied in the field of molecular detection, can solve the problems of low virus resistance, low sensitivity, and low success rate, and achieve the effects of low cost and high sensitivity
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[0030] Example 1: Design and screening of primers
[0031] The FHV-1C-27 strain gene (sequence FJ478159) registered in GenBank was compared by MegLign in DNAStar software, using Primer Premier 5.0 software, PCR primers and Taq man probe sequences, using fluorescent groups as the luminescent groups of the probes , using different wavelength bands of the corresponding fluorescent quantitative PCR instrument for detection. Design and synthesize a pair of FHV specific primers P1 / P2. The designed primers were analyzed as follows: interference between primers, hairpin structure and dimer. And through the BLAST function of NCBI to preliminarily identify its specificity. After screening, the primers with complementary sequences between the primers themselves and between the primers, and the single strand of the amplification product that can form a secondary structure were excluded, and the primers and probe sequences shown in Table 1 below were selected. The primers were synthesiz...
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