Method for improving interfacial catalysis reaction efficiency based on microcapsule prepared from natural pure protein

A catalytic reaction, protein technology, applied in the direction of enzyme stability, etc., can solve the problems of poor biocompatibility, reduce the stability of natural protein enzymes, etc., to achieve the effect of ensuring stability, good biocompatibility, and increasing the contact area

Active Publication Date: 2017-07-28
HARBIN INST OF TECH
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  • Claims
  • Application Information

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Problems solved by technology

[0004] In order to solve the problems of poor biocompatibility and reduced stability of natural protein enzymes in the existing water-in-oil system, the

Method used

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  • Method for improving interfacial catalysis reaction efficiency based on microcapsule prepared from natural pure protein
  • Method for improving interfacial catalysis reaction efficiency based on microcapsule prepared from natural pure protein
  • Method for improving interfacial catalysis reaction efficiency based on microcapsule prepared from natural pure protein

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specific Embodiment approach 1

[0017] Embodiment 1: A method of preparing microcapsules based on natural pure protein in this embodiment to improve the efficiency of interface catalytic reaction is carried out according to the following steps:

[0018] 1. Preparation of PBS buffer solution: NaH 2 PO 4 and Na 2 HPO 4 Mix and dissolve in deionized water to prepare PBS buffer solution; the NaH 2 PO 4 with Na 2 HPO 4 The mass ratio is 1:(25~46); the concentration of the PBS buffer solution is 45mmol / L~55mmol / L, and the pH value is 8.0~8.2;

[0019] 2. Preparation of natural pure protein solution: dissolve natural pure protein in the PBS buffer solution obtained in step 1 to obtain natural pure protein solution; the concentration of the natural pure protein solution is 0.5mg / mL~2mg / mL; The natural pure protein is one or a mixture of several of lysozyme, lipase and urease, and when the natural pure protein is a mixture, various substances are mixed in equal mass;

[0020] Three, the preparation of oil pha...

specific Embodiment approach 2

[0024] Specific embodiment two: the difference between this embodiment and specific embodiment one is: NaH described in step one 2 PO 4 with Na 2 HPO 4 The mass ratio is 1:40. Other steps and parameters are the same as those in the first embodiment.

specific Embodiment approach 3

[0025] Embodiment 3: This embodiment differs from Embodiment 1 or Embodiment 2 in that: the concentration of the PBS buffer solution in step 1 is 50 mmol / L. Other steps and parameters are the same as those in Embodiment 1 or 2.

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Abstract

The invention belongs to the field of biological catalytic synthesis, and particularly relates to a method for improving interfacial catalysis reaction efficiency based on a microcapsule prepared from natural pure protein. The method aims to solve the problems that an existing water-in-oil system is poor in biocompatibility and enzyme stability of pure protein is reduced. The method includes the steps: first, preparing natural pure protein solution; second, preparing oil-in-water microemulsion with natural protein serving as a stabilizer by a Pickering microemulsion method; third, preparing the natural protein microcapsule by taking the microemulsion as a template, and improving the catalysis efficiency of oil-water solution by the aid of catalytic activity of the natural protein. The natural protein serves as the stabilizer, the complicated process of synthesizing the stabilizer is avoided, and efficient oil-water catalysis is realized.

Description

technical field [0001] The invention belongs to the field of biocatalytic synthesis, and in particular relates to a method for preparing microcapsules based on natural pure protein to improve the efficiency of interface catalytic reaction. Background technique [0002] Enzyme is a natural pure protein with catalytic activity, and its catalytic reaction has many advantages, such as fast reaction rate, good stereoselectivity, mild reaction conditions and so on. Therefore, the use of enzyme-catalyzed reactions for the synthesis and transformation of substances is a hot area of ​​cross-research in biosynthesis and chemosynthesis. In the actual reaction system, how to maintain the activity of the enzyme during the reaction and improve the catalytic efficiency of the enzyme has become the research focus of the enzyme-catalyzed reaction. Since enzyme activity depends on the medium or microenvironment in which it exists, it has always been a research problem for scientists to explo...

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Application Information

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IPC IPC(8): C12N9/96
CPCC12N9/96
Inventor 王磊刘小曼周玉婷黄鑫
Owner HARBIN INST OF TECH
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