Target fluorescent dye and application thereof
A fluorescent dye, targeting technology, used in luminescent materials, preparations for in vivo experiments, medical preparations with non-active ingredients, etc., can solve problems such as damage and normal tissue damage, and achieve excellent performance and high drug loading capacity. , good chemical stability and biocompatibility
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preparation example Construction
[0061] More specifically, the preparation method includes the following steps:
[0062] (1) Preparation of drug-loaded hydroxyapatite (D@HAP);
[0063] (2) Surface modification of drug-loaded hydroxyapatite with targeted fluorescent dyes.
[0064] In a further specific embodiment, the above step (1) is to prepare drug-loaded hydroxyapatite (D@HAP) by reacting water-soluble calcium salt, phosphate and drug in an ethanol-water system at room temperature. Wherein, the soluble calcium salt is preferably but not limited to Ca(NO 3 ) 2 4H 2 O; the soluble phosphate is preferably but not limited to (NH 4 )2 HPO 4 .
[0065] On the other hand, the drug mentioned in the drug-loaded hydroxyapatite is selected from: doxorubicin, paclitaxel, cisplatin, gemcitabine, fluorouracil, Gaino, epirubicin, cyclophosphamide, vincristine, Bray Mycin, dopafil or its derivatives. Especially preferred is doxorubicin (DOX), doxorubicin hydrochloride (DOX·HCl) or derivatives thereof. It can be e...
Embodiment 1
[0074] Example 1. DFA 1 Preparation of @DOX@HAP
[0075] 1.1 Synthesis of drug-loaded hydroxyapatite (DOX@HAP)
[0076] Combined by precipitation hydrothermal method, weigh 30mg of doxorubicin hydrochloride, 1.490g of Ca(NO 3 ) 2 4H 2 O and 0.495g of (NH 4 ) 2 HPO 4 respectively dissolved in 2mL of ultrapure water, 30mL of absolute ethanol and 2mL of ultrapure water, and Ca(NO 3 ) 2 4H 2 The pH of the ethanol solution of O was adjusted to about 10.5. In this alkaline environment, slowly add an aqueous solution of doxorubicin hydrochloride dropwise while stirring, and after stabilizing for 10 minutes, continue to add dropwise (NH 4 ) 2 HPO 4 Aqueous solution, after the dropwise addition, continue to stir for 1 hour, then pour the turbid solution into the reaction kettle, react at room temperature at 25°C for 72 hours, then centrifuge at high speed, wash the product with ultrapure water and absolute ethanol several times, vacuum the product at 60°C Dry for 12 hours ...
Embodiment 2
[0079] Example 2. Testing of basic properties of drug-loaded hydroxyapatite surface-modified targeting fluorescent dyes
[0080] 2.1 Measurement of the size and morphology of drug-loaded hydroxyapatite with surface-modified targeting fluorescent dyes
[0081] The morphology and size of the drug-loaded hydroxyapatite with surface-modified targeting fluorescent dye obtained in Example 1 was determined by observation with a transmission electron microscope. The test results are shown in figure 1 . It can be clearly seen from the transmission electron microscope pictures that the DFA synthesized by co-precipitation 1 @DOX@HAP nanoparticles are rod-shaped, with an average length of 60-130 nm and an average diameter of 15-50 nm. They are not easy to aggregate and have good dispersion.
[0082] 2.2 Advanced Fourier Transform Infrared Spectroscopy
[0083] Take 1-2 mg drug-loaded hydroxyapatite sample of surface-modified targeting fluorescent dye, mix it with dry potassium bromide...
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