Bacteriophage for preventing and treating prawn vibrio parahaemolyticus disease and expanding culture method thereof

Abstract

The invention discloses a bacteriophage for preventing and treating a prawn vibrio parahaemolyticus disease and an expanding culture method thereof. Vibrio parahaemolyticus infected by South American white prawn are used for successfully screening out a strong lytic bacteriophage in seawater, titer of the bacteriophage is up to 1010 to 1012pfu / mL, fermentation period is 8 to 12h, treatment effects are obvious, and the bacteriophage for preventing and treating the prawn vibrio parahaemolyticus disease is of great significance for treatment of diseases causes by the vibrio parahaemolyticus and development of bacteriophage microecological preparations. A storage unit code of the bacteriophage is China Center for Type Culture Collection, address is China Center for Type Culture Collection in Wuhan University Campus, Wuchang Luojia Mountain, Wuhan City, preserved date is December 9, 2016, preservation number is CCTCC NO: M 2016740, a preservation test result is survival, name is vibrio parahaemolyticus bacteriophage VP11, and classification is named as Vibrio parahaemolyticus bacteriophage VP11.

Description

technical field [0001] The invention belongs to the field of biotechnology, and relates to a phage used for preventing and treating Vibrio parahaemolyticus and its expansion method. Background technique [0002] At present, the main means of controlling and eliminating pathogenic bacteria are still various physical or chemical methods, including the use of various antibiotics. Both physical and chemical methods have obvious disadvantages. First of all, the elimination effect of physical methods on various pathogenic bacteria can only be applied to a certain link in the related field, and cannot be applied to all links in the entire field. Secondly, although the acid-base treatment in the chemical method can achieve a certain control effect, However, after harmful bacteria form biofilms, the effects of these methods are extremely unsatisfactory; finally, although antibiotics are effective at the beginning, long-term use will not only enhance the drug resistance of pathogenic...

AI Technical Summary

Problems solved by technology

First of all, the elimination effect of physical methods on various pathogenic bacteria can only be applied to a certain link in the related field, and cannot be applied to all links in the entire field. Secondly, although the acid-base treatment in the chemical method can achieve a certain control effect, However, after harmful bacteria form biofilms, the effects of these methods are extremely unsatisfactory; finally, although antibiotics are effective at the beginning, long-term use will not only enhance the drug resistance of pathogenic bacteria, but also inhibit beneficial bacteria, and ultimately cannot effectively control , eliminate pathogenic bacteria

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