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Ophiocordyceps xuefengensis stroma and artificial cultivation method thereof

A technology of artificial cultivation and sub-block, applied in the field of Xuefeng Cordyceps sub-block and its artificial cultivation, can solve the problems of the decline in the quantity and quality of Cordyceps, the destruction of the ecological environment, the scarcity of supply, etc. low effect

Active Publication Date: 2017-08-04
湖南省中医药研究院
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] However, due to years of excavation of wild Cordyceps and the continuous destruction of its ecological environment, the quantity and quality of Cordyceps have dropped significantly. The supply of goods is scarce and the price is high, which is far from meeting the growing demand of the market.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] A kind of Xuefeng Cordyceps subunit, slightly cylindrical, often pointed or blunt at the top, unbranched or with short branches at the top, light dark yellowish brown.

[0027] A method for artificially cultivating Cordyceps xuefeng, the method steps are as follows:

[0028] 1) Preparation of mother species: Tissue separation of Cordyceps xuefeng fungus, the inoculation amount of 2 mm length of the subsea was inserted into the slant medium, at 25 ° C, the relative air humidity was 60%, and dark culture was carried out for 15 days until the hyphae were overgrown Inclined surface, pick the inclined surface with developed basal hyphae and turn yellow as the mother species.

[0029] Preparation of slant medium: add 200g of potato filtrate, 20g of sucrose, 2g of peptone, KNO to each 1000mL medium 3 1g, KH 2 PO 4 1g, MgSO 4 ·7H 2 O0.5g, the water of agar 15g and surplus. Peel the potatoes and cut into small strips, add water and boil for about 20 minutes until the potat...

Embodiment 2

[0037] A kind of Xuefeng Cordyceps subunit, slightly cylindrical, often pointed or blunt at the top, unbranched or with short branches at the top, light dark yellowish brown.

[0038] A method for artificially cultivating Cordyceps xuefeng, the method steps are as follows:

[0039] 1) Preparation of mother species: Tissue separation of Cordyceps xuefeng fungus, the inoculum of 3 mm in length of the subsea was inserted into the slant medium, at 28 ° C, the relative air humidity was 90%, and dark culture was carried out for 25 days until the hyphae were overgrown Inclined surface, pick the inclined surface with developed basal hyphae and turn yellow as the mother species.

[0040] Preparation of slant medium: add 200g of potato filtrate, 20g of sucrose, 2g of peptone, KNO to each 1000mL medium 3 1g, KH 2 PO 4 1g, MgSO 4 ·7H 2 O0.5g, the water of agar 20g and surplus. Peel the potatoes and cut into small strips, add water and boil for about 20 minutes until the potatoes are...

Embodiment 3

[0048] A kind of Xuefeng Cordyceps subunit, slightly cylindrical, often pointed or blunt at the top, unbranched or with short branches at the top, light dark yellowish brown.

[0049] A method for artificially cultivating Cordyceps xuefeng, the method steps are as follows:

[0050] 1) Preparation of the mother species: Tissue separation of the wild sporozoites, the inoculation amount of the sporozoites with a length of 2 mm was inserted into the slant medium, and cultured in the dark at 26° C. with a relative air humidity of 68% for 17 days until the hyphae covered the slant. , pick the slant with well-developed basal hyphae and turn yellow as the mother species.

[0051] Preparation of slant medium: add 200g of potato filtrate, 20g of sucrose, 2g of peptone, KNO to each 1000mL medium 3 1g, KH 2 PO 4 1g, MgSO 4 ·7H 2 O0.5g, the water of agar 16g and surplus. Peel the potatoes and cut into small strips, add water and boil for about 22 minutes until the potatoes are soft b...

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Abstract

The invention relates to an ophiocordyceps xuefengensis stroma and an artificial cultivation method thereof. The method comprises the following steps: performing tissue separation for a culture, a wild culture or a stroma obtained after successful cultivation of the culture, inoculating into a slant culture medium, preparing a slant mother strain, then inoculating the slant mother strain into a liquid culture medium, preparing a primary culture, inoculating the primary culture into the liquid culture medium to prepare a spawn, and culturing the spawn in a culture medium for production to obtain the artificially-cultivated ophiocordyceps xuefengensis stroma. The ophiocordyceps xuefengensis stroma and the artificial cultivation method thereof have advantages of simplicity in operation, low cost, experimental reproducibility and the like, are suitable for mass production and have wide development prospect.

Description

technical field [0001] The invention relates to a method for artificially cultivating Cordyceps sinensis, in particular to Cordyceps xuefeng and its artificial cultivation method. Background technique [0002] Cordyceps xuefeng is the fungus Ophiocordyceps xuefengensis T.C.Wen, R.C.Zhu, J.C.Kang & K.D.Hyde of the family Cordyceps, a dry complex or a dry complex of sub-seats and larval corpses that parasitize on the larvae of bat moths. The dry larval corpses (sclerotia) of mycelia are mainly distributed in Dongkou County and Longhui County, Hunan Province, and grow in the near-ground roots or stems of the plant Verbenaceae Clerodendrum cyrtopphyllum Turcz. at an altitude of 300-1000m. Cordyceps Xuefeng has the effects of nourishing the lungs and kidneys, relieving cough and resolving phlegm, and it is mainly used to treat kidney deficiency, impotence, nocturnal emission, sore waist and knees, chronic cough and dyspnea. Modern scientific research shows that many types of Cor...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/14C12R1/645
CPCC12N1/14
Inventor 张水寒钟灿金剑黄惠勇陈作红谢昭明朱如彩刘浩蔡媛
Owner 湖南省中医药研究院
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