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A kind of cell membrane modified nano carbon sphere material and its preparation method and application

A technology of nano-carbon spheres and cell membranes, applied in material separation, analysis materials, instruments, etc., can solve the problems of low adsorption capacity of cell membrane-silica gel materials, unfavorable popularization of cell membrane separation materials, complicated procedures, etc., to reduce screening time and experiment Cost, cheap effect

Active Publication Date: 2019-11-08
TONGDE HOSPITAL OF ZHEJIANG PROVINCE
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  • Application Information

AI Technical Summary

Problems solved by technology

Cell membrane chromatography technology provides a new idea and method for the screening of active ingredients of traditional Chinese medicine, but due to the following disadvantages: (1) The concentration of Chinese medicine ingredients varies greatly, but the adsorption capacity of the cell membrane-silica gel material is very low, and it is easy to saturate and adsorb high concentrations ingredients, but it is difficult to identify most low-concentration active ingredients; (2) the operation is complicated
This process requires more equipment and complicated procedures, which is not conducive to the popularization of cell membrane separation materials

Method used

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  • A kind of cell membrane modified nano carbon sphere material and its preparation method and application
  • A kind of cell membrane modified nano carbon sphere material and its preparation method and application
  • A kind of cell membrane modified nano carbon sphere material and its preparation method and application

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Embodiment Construction

[0034] 1) Preparation of carbon spheres

[0035] Weigh 10g of glucose, dissolve it in 200ml of distilled water, stir for 10 minutes, transfer the glucose solution into a polytetrafluoroethylene-lined hydrothermal kettle (filling degree is about 80%), put the hydrothermal kettle into the electric heating constant temperature blast drying In the box, hydrothermal reaction was carried out at a constant temperature of 200°C for 10 hours. Then cool naturally to room temperature to obtain a brown suspension, centrifuge to collect the generated carbon spheres, wash with deionized water and absolute ethanol three times, and finally dry at 80°C for 6 hours to obtain template carbon spheres, grind and set aside .

[0036] 2) Preparation of cell membrane suspension

[0037] When using osteoblasts to prepare cell membrane suspension, first take 0.1-0.5% trypsin protein and digest it to a density of 10 8For osteoblasts, centrifuge the cell suspension at 5000rpm for 10-30min, add 10mL of...

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Abstract

The invention relates to a drug screening material, in particular to a cell membrane modified nano carbon sphere material, and an application research of the nano carbon sphere material for screening active ingredients of traditional Chinese medicine. The cell membrane modified nano carbon sphere material is prepared from cell membrane modified nano carbon spheres. The material has good absorption capacity, not only can play a selective preservation effect of cell membrane spectrum, but also can greatly increase the absorption amount of active ingredients, and provides an experimental basis to the further pharmacological activity verification.

Description

technical field [0001] The invention relates to a drug screening material, in particular to a nano-carbon sphere material modified by cell membranes, and its application research for screening active ingredients of traditional Chinese medicines. Background technique [0002] The screening of active ingredients of traditional Chinese medicine is the original work of innovative drug research. Due to the integrity of the action of traditional Chinese medicine, the complexity of the chemical composition and mechanism of action of traditional Chinese medicine, the research on the material basis of the efficacy of traditional Chinese medicine has slowed down, which has become one of the bottlenecks restricting the modernization of traditional Chinese medicine. For a long time, the screening of active ingredients of traditional Chinese medicine has basically borrowed the idea of ​​research on active ingredients of natural products, that is, to separate and identify the chemical com...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N30/06G01N30/60
CPCG01N30/06G01N30/6052
Inventor 王娜妮王绪平吴人杰寿旦
Owner TONGDE HOSPITAL OF ZHEJIANG PROVINCE