Solenopsis invicta contact insecticide and application of fipronil in eliminating solenopsis invicta
A technology of red fire ants and insecticides, applied in the direction of insecticides, applications, biocides, etc., can solve the problems of less research and achieve the effects of simple preparation, good killing effect, and convenient use
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Embodiment 1
[0021] There are many control methods for RIFA, among which chemical control technology is the most effective, deepest and most widely used control method. Spreading bait is the most effective and long-lasting way to control RIFA in a large area, and mound drench is also one of the effective ways to control RIFA in a timely, effective and rapid manner. This application selects 7 different types of insecticides to test the contact toxicity of RIFA to clarify the toxicity difference of different pesticides to RIFA and the sensitivity difference of different grades of RIFA (queen and worker ants) to pesticides.
[0022] experiment material
[0023] Test insects and materials
[0024] The tested RIFAs were collected from Zengcheng, Guangzhou, Guangdong Province and the campus of South China Agricultural University. Referring to the method of Kuriachan et al. (2000), the RIFA colony was collected from the field in a large plastic bucket and placed for 1-2 days. After the ant colo...
Embodiment 2
[0082] Cytochrome P450 (Cytochrome P450, P450 for short) is the core part of the multifunctional oxidase system, which plays a role in terminal oxidation in the oxidative metabolism of the entire enzyme system (Qiu Lihong et al., 1999; Isin and Guengerich, 2007). P450 can participate in the metabolism of various exogenous substances and endogenous substances in insects. The following methods are commonly used for the determination of cytochrome P450 activity: 1. O-deethylation method: the cytochrome P450 enzyme is characterized by the activity of 7-ethoxycoumarin O-deacetylase (ECOD) system activity. After deethylation of 7-ethoxycoumarin (7-ethoxycoumarin) into 7-hydroxycoumarin (7-hydroxycoumarin), the fluorescence value can change (excitation wavelength 368nm, emission wavelength 456nm), using fluorescence spectroscopy Photometer, the change of fluorescence value produced by measuring the amount of 7-hydroxycoumarin is used to characterize the activity of cytochrome P450 (...
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