A fluorescent probe for detecting cysteine ​​and its preparation method and application

A fluorescent probe, cysteine ​​technology, applied in the field of fluorescent detection, can solve the problems of low sensitivity, unreported fluorescent probes, changes, etc., and achieve the effect of broad application prospects

Active Publication Date: 2019-08-20
XUCHANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] Capillary electrophoresis is an electrophoretic separation analysis method that uses an elastic quartz capillary as the separation channel, uses a high-voltage direct current electric field as the driving force, and realizes separation according to the differences in the mobility and distribution behavior of the components in the sample. The capillary diameter is small, so that The optical path is too short, the sensitivity is low, and the electroosmosis will change due to the composition of the sample, which will affect the reproducibility of the separation. These disadvantages will lead to errors in disease diagnosis;
[0008] The HPLC method has high accuracy and simple sample processing, but it is limited by the chromatographic column and takes a long time
[0009] The fluorescent probe detection method has attracted the attention of researchers for its advantages of non-destructive and visualized in situ detection, but no fluorescent probes that can specifically respond to cysteine ​​have been reported yet.

Method used

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  • A fluorescent probe for detecting cysteine ​​and its preparation method and application
  • A fluorescent probe for detecting cysteine ​​and its preparation method and application
  • A fluorescent probe for detecting cysteine ​​and its preparation method and application

Examples

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Embodiment 1

[0057] A fluorescent probe for detecting cysteine, its synthesis route is as follows:

[0058]

[0059] Concrete preparation method comprises the following steps:

[0060] (1) Get 4-bromo-1,8-naphthalene dicarboxylic anhydride and n-butylamine to react to obtain compound 1, and its structural formula is as follows:

[0061]

[0062] The specific synthesis method of the step (1) is as follows: 4-bromo-1,8-naphthalene dicarboxylic anhydride and n-butylamine with a molar ratio of 1:6 are added to ethanol, and the reaction is refluxed under nitrogen protection until the reaction liquid is clear , concentrated and cooled to precipitate crystals, and recrystallized from ethanol to obtain the compound 1.

[0063] (2) Using copper sulfate as a catalyst, the compound 1 obtained in step (1) is reacted with sodium methylate to obtain compound 2, and its structural formula is as follows:

[0064]

[0065] The specific synthesis method of the step (2) is as follows: take the com...

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PUM

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Abstract

The invention provides a fluorescence probe for detecting cysteine and a preparation method and application thereof. The structural formula of the fluorescence probe is as shown in the specification. The fluorescence probe realizes specific recognition and response of cysteine, and is not affected by interfering ions; and moreover, whether the cysteine exists or not can be directly judged by people with naked eyes, and the fluorescence probe has wide application prospect in the field of detection of biological molecules.

Description

technical field [0001] The invention belongs to the technical field of fluorescence detection, and in particular relates to a fluorescent probe for detecting cysteine, a preparation method and application thereof. Background technique [0002] Cysteine ​​is an amino acid with physiological functions, and it is the only amino acid with a reducing group sulfhydryl (-SH) among more than 20 kinds of amino acids that make up proteins. Medical research has shown that cysteine ​​is related to many diseases, such as renal failure, Alzheimer's disease, etc., and their content changes in organisms can be used as the basis for the diagnosis of these diseases, so a detection method for selectively detecting cysteine ​​has been developed especially important. [0003] At present, there are many methods for determining amino acid content, such as colorimetric method, iodometric method, enzyme cycle method, capillary electrophoresis method and HPLC method, etc. These methods have their ow...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07D405/06C09K11/06C09B57/08G01N21/64G01N21/78
Inventor 侯旭锋许志红李云强李振升栗剑云
Owner XUCHANG UNIV
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