Typing detection method and kit for high-risk human papilloma virus (HPV)

Abstract

The invention provides a typing detection method and kit for a high-risk human papilloma virus (HPV). According to the method, a method for performing fluorescence nucleic acid amplification by adopting multiple pairs of primers and multiple probes is combined with fusion curve analysis, so that multiple target nucleic acid sequences can be detected in a single-wavelength fluorescence channel; an L1 segment sequence of an HPV genome is used as a target sequence for designing an HPV specific primer, a TaqMan probe and a PCO hybridization probe which are not completely complementary with the TaqMan probe, and a 3' end of the hybridization probe can be subjected to fluorescence quenching group labeling or non-fluorescence quenching group labeling according to purposes and cannot be extended after the hybridization probe is labeled, so that an HPV gene typing kit containing a primer probe and other fluorescence PCR components is researched; and by combining fluorescence PCR amplification with single-wavelength fusion analysis, the types 16, 18, 31, 52 and 59 of the HPV can be identified.

Description

technical field [0001] The invention relates to a high-risk human papilloma virus typing detection method and kit, which belong to the field of biotechnology. Background technique [0002] Human papillomavirus (Human papillomavirus, HPV) belongs to the Papillomaviridae family, is a small molecule, non-encapsulated, circular double-stranded DNA virus, the genome length is about 8000 base pairs (bp), divided into 3 There are three functional regions, namely early transcribed region (E region), late transcribed region (L region) and non-transcribed region (long control region, LCR). HPV infects humans through direct or indirect contact with contaminated objects or through sexual transmission. The virus is not only host-specific, but also tissue-specific. It can only infect human skin and mucosal epithelial cells, and can cause squamous epithelial proliferation of human skin and mucous membranes. More than 130 kinds of papillae that cause human skin have been isolated so far. ...

AI Technical Summary

Problems solved by technology

Single-probe real-time quantitative PCR, because each probe is designed for a specific target site, with high specificity, each probe can only detect one target sequence, if multiple target sequences need to be detected, Multiple probes need to be added. Due to the limitation of the absorption wavelength of the fluorescent label of the probe, the types of gene sequence detection are restricted.

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