Seed sterilization method during aseptic seeding of orchids

Abstract

The invention discloses a seed sterilization method during aseptic seeding of orchids. The method comprises a sterilization method for non-cracked fruit pods and a seed sterilization method for cracked fruit pods. The method specifically comprises the following steps: removing persitent perianth, gynandrium and carpopodium of the fruit pods when the pods are not cracked, cleaning with running water, disinfecting in a 5% sodium hypochlorite solution for 20 minutes, disinfecting the surface with 75% alcohol for 3 minutes, and directly firing on an alcohol lamp to remove alcohol on the surface of the pods so as to complete disinfection; and placing the seeds in a 300-mesh nylon mesh bag with the size of 3cm*4cm when the fruit pods are cracked, performing hot melting and sealing, placing the nylon mesh bag in a 10.0ml of centrifuge tube, adding a disinfectant into the centrifuge tube so as to enable the liquid level of the disinfectant to immerse the nylon mesh, standing for 20-40 minutes, and flushing with sterile water for several times until disinfection is completed. The disinfection time can be effectively controlled, the surface disinfection effect is guaranteed, the operation is simple and convenient, the seed vigor is not influenced, and the method is a disinfection method suitable for orchid seeds and similar tiny seeds.

Description

technical field [0001] The invention relates to a method for sterilizing seeds when plants are sown, in particular to a method for sterilizing seeds when aseptically sowing orchid plants. Background technique [0002] The fruit of Orchidaceae is a capsule, which cracks and releases seeds after ripening under natural conditions. Since the capsule is a closed structure, its seeds are clean and sterile when they are not cracked, and the pods can be directly sterilized on the surface, and the seeds inside can be directly used for aseptic germination. However, in the process of hybrid breeding of orchids, due to negligence, the fruit pods cannot be harvested in time, and often encounter the phenomenon that the fruit pods will crack as soon as the maturity period arrives, or the orchid fruit pods will crack during storage and transportation. Once the cracked seeds are easily damaged Pollution. [0003] Because the seeds of orchids are very small and light in weight, conventional...

AI Technical Summary

Problems solved by technology

However, in the process of hybrid breeding of orchids, due to negligence, the fruit pods cannot be harvested in time, and often encounter the phenomenon that the fruit pods will crack as soon as the maturity period arrives, or the orchid fruit pods will crack during storage and transportation. Once the cracked seeds are easily damaged Pollution

[0003] Because the seeds of Orchidaceae are very small and light in weight, conventional surface disinfection methods are relatively complicated and the disinfection effect is poor, which may easily cause problems such as seed loss and uneven disinfection time, which in turn affects the success rate of aseptic germination.

There have been several studies on the experimental technology for surface disinfection of tiny seeds, but there is no research on the appropriate disinfection method for orchid seeds