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Application of grifolic acid in preparing drug for treating osteosarcoma

A technology of osteosarcoma and ash folic acid, applied in the application field of medicine, to achieve the effect of inhibiting growth, high killing effect, and low toxic and side effects

Inactive Publication Date: 2017-08-18
XIAN MEDICAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although gray folic acid can kill tumor cells by inhibiting the generation of ATP in cells, it is still unknown whether gray folic acid has the effect of killing tumors in vivo and whether it has obvious toxic side effects

Method used

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  • Application of grifolic acid in preparing drug for treating osteosarcoma
  • Application of grifolic acid in preparing drug for treating osteosarcoma
  • Application of grifolic acid in preparing drug for treating osteosarcoma

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Embodiment Construction

[0018] The present invention will be further described in detail below in conjunction with specific examples, the application of gray folic acid in the preparation of medicaments for treating osteosarcoma.

[0019] 1. Cell culture: A variety of osteosarcoma cells derived from humans and mice were used to detect the effect of ash folic acid. The human MG-63 cells, human U-2OS cells, human Saos-2 cells and mouse K7M2 cells used in the present invention are all used for detection. All the above cells were cultured in DMEM medium containing 10% fetal bovine serum at 37°C in 5% CO 2 Cultivate in an incubator, replace the culture medium every 2-3 days, and subculture after the cells grow to 80%. Passage was digested with 0.25% trypsin + 0.02% EDTA, and passaged according to the ratio of 1:3-5. Culture fluid, serum and digestive enzymes were all produced by Gibco.

[0020] 2. Cell activity detection: All the above-mentioned cultured cells were planted in 96-well culture plates. Af...

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Abstract

The invention discloses application of grifolic acid in preparing drug for treating osteosarcoma. Concentration of the grifolic acid in the drug is not less than 50umol / L, an administration mode of the drug is osteosarcoma intra-tumor injection, and injection dosage of the grifolic acid in each cubic millimeter of osteosarcoma is 0.1ml. The invention provides new application of the grifolic acid. The new application is found and proved on the basis of new functions of the grifolic acid. Through intra-tumor injection of the grifolic acid, osteosarcoma cells can be killed and damaged effectively, growth of osteosarcoma can be inhibited remarkably, and no obvious side effect appears. Due to strong killing and damaging effect on osteosarcoma and low toxic and side effect on organism, the grifolic acid is made good drug for treating tumors like osteosarcoma.

Description

technical field [0001] The invention belongs to the technical field of gray folic acid application, and in particular relates to the application of gray folic acid in the preparation of medicine for treating osteosarcoma. Background technique [0002] Osteosarcoma mostly occurs in young people and children, with a high degree of malignancy and a poor prognosis. Despite continuous improvements in clinical treatment, the survival rate of osteosarcoma has not improved further in the past three decades. Chemotherapy is the most important treatment for osteosarcoma besides surgery. Although increasing the dose of chemotherapy drugs can achieve a more significant effect of killing tumor cells, but the current chemotherapy drugs used in high doses produce unbearable side effects for patients , which limits its application in killing tumor cells. Therefore, finding safer and more effective anticancer drugs is of great significance. [0003] Grifolic acid is a natural product extr...

Claims

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Application Information

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IPC IPC(8): A61K31/192A61P35/00
CPCA61K31/192
Inventor 赵玉峰胡运生苏兴利梁向艳魏兰兰刘英光陈镝谢荣赵妍妍
Owner XIAN MEDICAL UNIV