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A kind of phellinus strain and its application

A Phellinus and mulberry technology, applied in the fields of application, fungi, plant cultivation, etc., can solve the problems that are difficult to control manually, and achieve the effect of good color and high yield

Active Publication Date: 2020-10-16
山东省菇园食用菌科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the artificial cultivation methods of Phellinus japonica mostly use the sawdust crushed from the branches of broad-leaved trees such as mulberry, poplar, and eucalyptus as the main cultivation raw materials, and the ground yellowing is mainly grown in bags in greenhouses. It is difficult to control the favorable (temperature, humidity, light, air) environment of Phellinus chinensis, and it is difficult to effectively ensure that the fruiting bodies of Phellinus phellidis grow out to achieve the effect of coexistence of quality and weight

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0041] Embodiment 1, the isolation and identification of Phellinus phylloxera strain GY-S01

[0042] 1. Obtaining of strain GY-S01

[0043] The fruiting body of wild Phellinus radix was collected from an ancient mulberry tree in the thousand-year-old mulberry forest in the old course of the Yellow River in Xiajin County, Shandong Province. After tissue separation and repeated breeding, a strain was obtained, which was named strain GY-S01.

[0044] 2. Molecular identification of strain GY-S01

[0045] In order to accurately identify the strain GY-S01, a conserved sequence on its ITS was sequenced, and the sequence was searched for homologous sequences in the GenBank nucleic acid sequence database. It was found that its homology with multiple Phellinus species reached More than 99%. It was confirmed that the strain GY-S01 was Phellinus, and it was named Phellinus mulberry GY-S01.

[0046] 3. Preservation of Phellinus strain GY-S01

[0047] Sanghuangporus sanghuang GY-S01 was...

Embodiment 2

[0048] The artificial cultivation of embodiment 2, Phellinus phylloxera strain GY-S01

[0049] 1. Breeding of mother species

[0050] Inoculate the mycelia of the Phellinus phylloxera strain GY-S01 into the slant of the mother seed culture medium, and culture it at 25° C. in the dark for 7-10 days (the slant medium is overgrown with bacteria).

[0051] 2. Cultivation of original species

[0052] Take the mother species mycelium cultivated in step 1 (two pieces the size of bean grains), insert it into the strain bottle containing the original seed medium, and cultivate it in the dark at 25°C for 10-20 days (the culture medium in the bottle is full of bacteria) .

[0053] 3. Cultivation of cultivars and yellowing cultivation

[0054] 1. Cultivation of cultivars

[0055]Take the original seed cultivated in step 2, insert it into a cultivation bag with a cultivation medium, seal the cultivation bag, and cultivate it at 25°C, 60-65% relative humidity, and dark conditions until ...

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Abstract

The invention discloses a phellinus strain and application thereof. The invention provides a mulberry tree Phellinus (Sanghuangporus sanghuang) GY‑S01, the preservation number of which is CGMCC No. 13579. Sanghuangporus sanghuang (Sanghuangporus sanghuang) GY‑S01, its characteristics are as follows: fast yellowing, it only takes about 20 days from the introduction of the fungus into the cultivation bag to the production of fruiting bodies, and the fruiting bodies can grow with a little environmental stimulation, which can realize The growth of mycelium and fruiting body proceeds synchronously. In a suitable environment, the fruiting body has good color and high yield, and it is an excellent variety for industrial cultivation of Phellinus radix fruiting body.

Description

technical field [0001] The invention relates to a phellinus strain and its application. Background technique [0002] Phellinus spp. is a precious perennial large medicinal fungus, usually born on the genus Morus (Morus L.). ", is a kind of precious Chinese herbal medicine, which is used in traditional Chinese medicine for the treatment of dysentery, night sweats, metrorrhagia, blood stranguria, astringent pain in the umbilical abdomen, prolapse of the anus, diarrhea, leukorrhagia, and amenorrhea. [0003] As experts at home and abroad research more and more on the anti-cancer mechanism and medicinal value of Phellinus Phellinus, Phellinus Phellinus has been widely recognized by people, and the demand for Phellinus Phellinus in the market is increasing. At present, the artificial cultivation methods of Phellinus japonica mostly use the sawdust crushed from the branches of broad-leaved trees such as mulberry, poplar, and eucalyptus as the main cultivation raw materials, and ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/14A01G18/40A01G18/00A01G18/20C12R1/645
CPCC12N1/145C12R2001/645
Inventor 邓玉福
Owner 山东省菇园食用菌科技有限公司