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Natural acaricide for mites, identification marker for mites, and method for manufacturing same

A technology for identifying markers and acaricides, applied in the direction of acaricides, botanical equipment and methods, and biocides, which can solve problems such as difficult control and imperfect acaricidal effect of longhorned blood ticks, and prevent secondary infection , Excellent acaricidal effect

Inactive Publication Date: 2017-08-18
IND COOP FOUND CHONBUK NAT UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, so far no vaccine or antiviral drug has been developed against fever with thrombocytopenia syndrome, so the best way is to control these vector pests themselves, but the hosts of the longhorned blood tick (wild rats, cats, birds, etc.) exist Diversity, and has the characteristics of being distributed in the whole region of Korea, so it is difficult to completely control
Therefore, there is an urgent need for management systems that can manage the emergence of the longhorned blood tick and control exposure, but the acaricidal effect of the long-horned blood tick has not been well studied

Method used

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  • Natural acaricide for mites, identification marker for mites, and method for manufacturing same
  • Natural acaricide for mites, identification marker for mites, and method for manufacturing same
  • Natural acaricide for mites, identification marker for mites, and method for manufacturing same

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0087] 1-1. Melissa essential oil extraction

[0088] The whole melissa plant used in the present invention is the product after washing and drying with distilled water, and after the grinding process. The ground melissa plant (100g) and 1200ml of distilled water were injected into a 3000ml round-bottomed flask and then continuously extracted by steam distillation extraction (SDE). The extraction conditions used in the present invention are as follows (the extraction temperature is 75° C., the extraction time is 5 hours, the extraction solvent is hexane, and the extraction volume is 200 ml). The above-mentioned melissa extract uses anhydrous magnesium sulfate, filter paper and a vacuum concentrator to remove melissa essential oil and impurities, and then is concentrated under reduced pressure and stored in the refrigerator.

[0089] 1-2. Preparation of Morinda officinalis extract

[0090] After washing Morinda officinalis 3 times with 1000 ml of distilled water, it was dried for mo...

Embodiment 2

[0092] 2-1. GC-MS analysis of melissa essential oil

[0093] In order to confirm the volatile composition contained in the melissa essential oil extracted in Example 1 above, 10 mg of melissa essential oil was dissolved in 1 ml of hexane and centrifuged. After filtering the supernatant, GC / MS was analyzed. The analysis conditions of GC / MS are as follows. The column is VF-5ms (30mm×0.25mm×0.25mm), the carrier gas (carrie rgas) is helium (1ml / min), the injection temperature is 250℃, the oven temperature is 50~250℃, and the rising temperature is Component analysis was performed under the conditions of 3℃, injection volume of 1μl, injection mode of split ratio of 20:1, using Mass Selective Detector (MSD), the mass range (mass range) was 28-550. Component analysis was performed under scan conditions. The results are shown in Table 1.

[0094] Table 1

[0095] GC-MS analysis results of melissa essential oil

[0096]

[0097] From Table 1, it was confirmed that the various compounds con...

Embodiment 3

[0110] Evaluation of the acaricidal activity of melissa essential oil extract against mites

[0111] (1) Experimental mites

[0112] The acaricidal effects of dust mites, house dust mites, tyrophagous mites and Haemaphysalis longhorne were tested. The test results of dust mites, house dust mites and tyrophagous mites were from the University of Agriculture and Life Sciences, Seoul National University on February 4, 2004 It was purchased in the laboratory of Professor An Yongjun from the Department of Agricultural Biology. In the past 10 years, without any acaricides, at a temperature of 26°C, a relative humidity of 70%, and no external environment, Raised. Haemaphysalis longhorned tick was collected around a reservoir in Wanju County, Jeollabuk-do, South Korea, and then tested for its acaricidal effect.

[0113] (2) Determination of acaricidal effect on experimental mites

[0114] In the determination of the acaricidal effect of the present invention, the fumigation method and the c...

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Abstract

The present invention relates to a natural acaricide having acaricidal activity against mites and an identification marker. More specifically, the present invention relates to a natural acaricide, an identification marker having an effect of identifying mites, and a method for manufacturing the same, the natural acaricide having excellent acaricidal activity effect against mites such as house dust mites and Haemaphysalis longicornis (murder mites) while using materials obtained by fractionizing and purifying essential oil of Melissa officinalis (lemon balm) or Morindae officinalis Radix extracts, which are natural ingredients, not synthetic materials.

Description

Technical field [0001] This application claims the priority of Korean patent application No. 10-2014-0 140038 filed on October 16, 2014 and Korean patent application No. 10-2015-01349 26 filed on September 23, 2015, both of which are References for this application. [0002] The present invention relates to a natural acaricide and / or identification mark for mites using a compound obtained by fractionating and purifying essential oil of Melissa officinalis or Morinda officinalis extract and its derivatives. Background technique [0003] Hygienic pests are distributed around human life and have always been regarded as the biggest headache. In particular, with regard to human diseases caused by vector pests of infectious diseases, since there is an epidemiological correlation between humans and vector pests, it is actually difficult to completely remove these pests. Therefore, in the field of sanitary pests, the development of methods to effectively control the vector pests of these...

Claims

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Application Information

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IPC IPC(8): A01N35/02A01N35/04A01N65/22A01N65/08A01P7/02
CPCA01N35/02A01N35/04A01N65/00A01N65/08A01N65/22Y02A50/30
Inventor 李会善梁智衍宋慈恩
Owner IND COOP FOUND CHONBUK NAT UNIV
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