Reagent box for detecting chromosome aneuploidy and preparing method and application thereof

Abstract

The invention relates to the technical field of biological detection, and relates to a reagent box for detecting chromosome aneuploidy and a preparing method and an application thereof. The reagent box comprises a first reagent, a third reagent with purification magnetic beads or a second reagent, and a third reagent with the purification magnetic beads; the first reagent comprises a breaking repairing buffer solution, a breaking repairing enzyme, a dATP, a dNTP, a DNA polymerase I and a rtaq enzyme; the second reagent comprises the breaking repairing buffer solution, the breaking repairing enzyme, the dATP, the dNTP, a klenow enzyme and the rtaq enzyme; the third reagent comprises T4 DNA ligase, a PNK buffer solution and a junction. According to the reagent box, through an efficient and precise building database method, chromosome abnormity is detected, the comparison rate is increased, the GC deviation is reduced, the dup rate is lowered, meanwhile 10bp fluctuation before sequencing can be reduced, and the situation of AT separation can be avoided.

Description

technical field [0001] The invention relates to the technical field of biological immune detection, in particular to a kit and its preparation method and application, in particular to a kit for detecting chromosomal aneuploidy and its preparation method and application. Background technique [0002] In my country, there are more than 50 million infertility patients. The incidence of habitual abortion is also increasing year by year. Clinically, the incidence of spontaneous abortion is 15% to 25%, and more than 80% of them are early abortions that occur before 12 weeks of pregnancy. Factors leading to spontaneous abortion include immune system abnormalities, thrombophilias, infections, genetic and epigenetic abnormalities, and other unexplained factors. Among the many known factors that cause spontaneous abortion, 50% to 60% of spontaneously aborted embryos are chromosomal abnormalities. For the two most important causes of early pregnancy abortion, abnormal chromosome numb...

AI Technical Summary

Problems solved by technology

Although traditional karyotype analysis is the gold standard for diagnosing chromosomal diseases, the operation is complicated and requires cell culture of aborted tissue, amniotic fluid cells, and villi tissue, which is prone to cell culture failure, missed detection, and misdiagnosis.

FISH does not require cell culture and is quick and easy, but it can only check the specific chromosome covered by the probe, and cannot fully reflect the chromosome status of abortion tissue, etc.

Although Array-CGH has the advantages of rapidity, strong specificity, and multiple detection sites, it is expensive and has not been widely used

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