Reagent box for detecting chromosome aneuploidy and preparing method and application thereof

A technology of aneuploidy and kits, applied in biochemical equipment and methods, DNA preparation, microbial measurement/testing, etc., can solve the problems of high price, complicated operation, misdiagnosis, etc., to reduce the dup rate and increase the ratio The effect of correct rate and reducing deviation

Pending Publication Date: 2017-08-22
MGI TECH CO LTD
View PDF3 Cites 4 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although traditional karyotype analysis is the gold standard for diagnosing chromosomal diseases, the operation is complicated and requires cell culture of aborted tissue, amniotic fluid cells, and villi tissue, which is prone to cell culture failure, missed detection, and misdiagnosis.
FISH does not require cell

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Reagent box for detecting chromosome aneuploidy and preparing method and application thereof
  • Reagent box for detecting chromosome aneuploidy and preparing method and application thereof
  • Reagent box for detecting chromosome aneuploidy and preparing method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0035] Example 1: Detection of CNV variation in abortion tissue samples

[0036] The database building process is as follows figure 1 shown, including the following steps:

[0037] 1. Sample collection and processing:

[0038] Eight abortion tissue samples were taken to extract DNA with QIAamp DNA Blood Mini Kit (QIAGEN), and finally the DNA was dissolved in 20 μL TE solution.

[0039] 2. End repair:

[0040] Configure the system according to Table 1.

[0041] Table 1

[0042]

[0043]

[0044] Add 10 μL of the reaction solution (Reagent I or Reagent II) into the DNA solution homogenized to 20 μL, mix well, and incubate at 37°C for 15 minutes; incubate at 65°C for 15 minutes, and cool down to 4°C at a rate of 0.1°C / sec.

[0045] 3. Joint connection:

[0046] The linker sequence used in this scheme is as follows (the sequence in this example is from 5' end to 3' end from left to right, " / / " indicates the modification group, "phos" indicates phosphorylation, B10 indi...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention relates to the technical field of biological detection, and relates to a reagent box for detecting chromosome aneuploidy and a preparing method and an application thereof. The reagent box comprises a first reagent, a third reagent with purification magnetic beads or a second reagent, and a third reagent with the purification magnetic beads; the first reagent comprises a breaking repairing buffer solution, a breaking repairing enzyme, a dATP, a dNTP, a DNA polymerase I and a rtaq enzyme; the second reagent comprises the breaking repairing buffer solution, the breaking repairing enzyme, the dATP, the dNTP, a klenow enzyme and the rtaq enzyme; the third reagent comprises T4 DNA ligase, a PNK buffer solution and a junction. According to the reagent box, through an efficient and precise building database method, chromosome abnormity is detected, the comparison rate is increased, the GC deviation is reduced, the dup rate is lowered, meanwhile 10bp fluctuation before sequencing can be reduced, and the situation of AT separation can be avoided.

Description

technical field [0001] The invention relates to the technical field of biological immune detection, in particular to a kit and its preparation method and application, in particular to a kit for detecting chromosomal aneuploidy and its preparation method and application. Background technique [0002] In my country, there are more than 50 million infertility patients. The incidence of habitual abortion is also increasing year by year. Clinically, the incidence of spontaneous abortion is 15% to 25%, and more than 80% of them are early abortions that occur before 12 weeks of pregnancy. Factors leading to spontaneous abortion include immune system abnormalities, thrombophilias, infections, genetic and epigenetic abnormalities, and other unexplained factors. Among the many known factors that cause spontaneous abortion, 50% to 60% of spontaneously aborted embryos are chromosomal abnormalities. For the two most important causes of early pregnancy abortion, abnormal chromosome numb...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
IPC IPC(8): C12Q1/68C12N15/10
CPCC12N15/1013C12N15/1093C12Q1/6883
Inventor 郭晶张艳艳钟娜张韶红赵霞江媛
Owner MGI TECH CO LTD
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap