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Method for identifying cold tolerance of rice
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An identification method and technology of cold tolerance, applied in the field of agricultural biology, can solve problems such as being easily affected by the environment, not being able to screen out cold-tolerant rice materials, and low seed setting rate
Inactive Publication Date: 2017-08-22
JILIN ACAD OF AGRI SCI
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Problems solved by technology
Artificial climate chamber treatment is not only costly, but also difficult to screen and identify large quantities of materials
In a word, the identification of cold tolerance is easily affected by the environment. Different environments (weather conditions) require different treatment conditions. If the treatment is too heavy, the rice pollen will be aborted, the seed setting rate will be too low, and the cold-tolerant rice materials will not be screened out; light, it will lead to high seed setting rate of rice, no difference between materials
[0005] Therefore, the traditional breeding method based on phenotypic identification cannot accurately understand the genetic basis of rice cold tolerance, so it is difficult to make accurate selection, and the progress of rice cold tolerance breeding is slow
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Embodiment 1
[0030] All test materials were sown in mid-April and transplanted on May 20. After 5 weeks of cultivation, plants with basically the same growth and development process were selected and transplanted into plastic buckets, with 3 plants per bucket.
[0031] When the rice plant has 3 tillers and just begins to joint, put the name and date on the signboard, move the test material into 30cm deep water (water temperature 19°C) for processing, and place the test material at a suitable temperature as a contrast. After 7 days of treatment, Moved into the greenhouse to grow naturally until maturity.
[0032] Before harvesting, measure ear neck length (ear extruding degree); After harvesting, every plant gets main ear, surveys its ear length, ear grain number, seed-setting rate; Experimental result is as table 1 and Figure 1~4 shown.
Embodiment 2
[0034] All test materials were sown in mid-April, transplanted on May 20, and cultivated for 5 weeks. Plants with basically the same growth and development process were selected and transplanted into plastic buckets, with 3 plants per bucket.
[0035] When the rice plant has 1 tiller just beginning to joint, put the name and date on the signboard, move the test material into 30cm deep water (water temperature 20°C) for processing, and place the test material at a suitable temperature as a control at the same time. After 7 days of treatment, Moved into the greenhouse to grow naturally until maturity.
[0036] Before harvesting, the ear neck length (ear extension degree) was measured; after harvesting, the main ear was taken from each plant, and the ear length, ear grain number, and seed setting rate were measured.
Embodiment 3
[0038] All test materials were sown in late April, transplanted on May 20, and cultivated for 4 weeks. Plants with basically the same growth and development process were selected and transplanted into plastic buckets, with 3 plants per bucket.
[0039] When the rice plant has 1 tiller and just starts jointing, put the name and date on the signboard, move the test material into 30cm deep water (water temperature 16°C) for processing, and place the test material at a suitable temperature as a control. After 7 days of treatment, Moved into the greenhouse to grow naturally until maturity.
[0040] Before harvesting, the ear neck length (ear extension degree) was measured; after harvesting, the main ear was taken from each plant, and the ear length, ear grain number, and seed setting rate were measured.
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Abstract
The invention discloses a method for identifying cold tolerance of rice, relates to the field of agricultural biotechnology, and especially relates to a method for identifying cold tolerance of rice by using low temperature treatment in a jointing stage of rice. The method for identifying cold tolerance of rice comprises the following steps: (1) rice plants whose growth and development processes are basically consistent are selected and transplanted into a plastic barrel; (2) low temperature treatment is carried out in a jointing stage; (3) cold tolerance of rice is evaluated. The cold tolerance of rice is identified by utilizing low temperature treatment in the jointing stage of rice for the first time, and difference between setting rate in low temperature treatment in jointing stage and setting rate in a natural condition is used for better reflecting cold tolerance of varieties; and at the same time, researches show that low temperature treatment in jointing stage and meiosis stage has the same influence result for setting rate, and has an important meaning for breeding rice with cold tolerance.
Description
technical field [0001] The invention relates to the field of agricultural biotechnology, in particular to a method for identifying the cold tolerance of rice by performing low-temperature treatment at the jointing stage of rice. Background technique [0002] Rice is an important food crop. More than half of the world's population uses rice as the main food, and it is planted all over the world. Rice is a temperature-loving crop, and its growth and development are very sensitive to temperature. Chilling damage occurs throughout the growth and development of rice from seed germination to maturity. Chilling injury at the germination stage will lead to delayed germination and reduced germination rate, hindering seedling formation; at the seedling stage, low temperature will delayseedling growth or even rotten seedlings and dead seedlings, resulting in poor colony formation and inconsistent maturity; Chilling damage that occurs during this period will cause poor development of...
Claims
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