Method for regulating and controlling fermentation efficiency of clostridium butyricum

A technology of fermentation efficiency and butyric acid bacteria, applied in the biological field, can solve the problems of unstable active ingredient content, unfavorable fermentation efficiency of butyric acid bacteria, complex organic nitrogen source components, etc., and achieves an increase in the number of viable cells of butyric acid bacteria and high controllability of operation. , the effect of simple ingredients

Active Publication Date: 2017-09-01
河南省南街村(集团)有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The purpose of the present invention is to provide a method for regulating the fermentation efficiency of butyric acid bacteria, which solves the problem that the composition of the organic nitrogen source is complex and the dosage is large, which makes the regulation cost of the fermentation process relatively high, and the content of the active ingredients in the organic nitrogen source is not fixed, which is not conducive to The Problem of Accurate Regulation of Butyric Acid Bacteria Fermentation Efficiency

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  • Method for regulating and controlling fermentation efficiency of clostridium butyricum
  • Method for regulating and controlling fermentation efficiency of clostridium butyricum

Examples

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Effect test

Embodiment 1

[0039] A method for regulating the fermentation efficiency of butyric acid bacteria, specifically comprising the following steps:

[0040] Step 1, preparation of culture medium

[0041] Prepare liquid culture medium, every liter of seed liquid medium is prepared according to the following formula: glucose 40g / L, peptone 50g / L, yeast powder 15g / L, dipotassium hydrogen phosphate 1.5g / L, magnesium sulfate heptahydrate 0.75g / L, Light calcium carbonate 1g / L, manganese sulfate monohydrate 0.02g / L, the balance is water, pH=7.3, deoxygenation and sterilization by conventional methods;

[0042] The anaerobic tube slant was prepared, and each liter of the anaerobic tube slant medium was prepared according to the following formula: 20 g of agar was added to 1 L of the seed liquid medium.

[0043] Step 2, prepare butyric acid bacteria seed solution

[0044] According to the requirements of aseptic and anaerobic operation, pick up the cells from the freeze-dried tube of butyric acid bact...

Embodiment 2

[0054] A method for regulating the fermentation efficiency of butyric acid bacteria, specifically comprising the following steps:

[0055] Step 1, preparation of culture medium

[0056] Same as Example 1

[0057] Step 2, prepare butyric acid bacteria seed solution

[0058] Same as Example 1

[0059] Step 3, fermentation culture

[0060] Except for the step of adding sodium formate solution, the rest of the fermentation conditions were the same as in Example 1.

[0061] Wherein, the addition condition of sodium formate solution is as follows:

[0062] Feed sterile, anaerobic sodium formate solution with a concentration of 10 g / L within 2 hours during the fermentation of butyric acid bacteria at a flow rate of 375 mL / h until the final concentration of sodium formate in the fermentation broth is 0.125 g / L.

[0063] Anaerobic fermentation 14h in the above-mentioned way, cell number (carry out colony CFU counting with Hungate rolling tube counting method) is 8.26 * 10 8 CFU / m...

Embodiment 3

[0065] A method for regulating the fermentation efficiency of butyric acid bacteria, specifically comprising the following steps:

[0066] Step 1, preparation of culture medium

[0067] Same as Example 1

[0068] Step 2, prepare butyric acid bacteria seed solution

[0069] Same as Example 1

[0070] Step 3, fermentation culture

[0071] Except for the step of adding sodium formate solution, the rest of the fermentation conditions were the same as in Example 1.

[0072] Wherein, the addition condition of sodium formate solution is as follows:

[0073] Feed a sterile, anaerobic sodium formate solution with a concentration of 10 g / L within 2 hours during the fermentation of butyric acid bacteria at a flow rate of 540 mL / h until the final concentration of sodium formate in the fermentation broth is 0.18 g / L.

[0074] Anaerobic fermentation 14h in the above-mentioned manner, cell number (carry out bacterial colony CFU counting with Hungate rolling tube counting method) is 8.12...

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Abstract

The invention discloses a method for regulating and controlling fermentation efficiency of clostridium butyricum. The method comprises a culture medium preparing stage, a clostridium butyricum seed solution preparing stage and a fermentation culture stage, wherein at the fermentation culture stage, a 10g / L sterile and oxygen-free sodium formate solution is added in 2 hours of fermentation of the clostridium butyricum in a fed-batch manner until the final concentration of sodium formate in fermentation broth is 0.0625g / L to 0.18g / L. According to the method, sodium formate serves as a metabolism regulating and controlling factor, the mechanism is clear, the ingredients are simple, the raw materials are readily available, the production cost is low, and the actual operation controllability is high, so that the method is applicable to large-scale fermentation production of the clostridium butyricum; in addition, the viable cell count of the clostridium butyricum during tank discharging is increased, and an excellent technical effect is generated and is obviously improved compared with the prior art.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to a method for regulating the fermentation efficiency of butyric acid bacteria. Background technique [0002] Butyric acid bacteria (Clostridium butyricum), as a new probiotic for humans or animals, has important functions such as adjusting the balance of intestinal flora, enhancing immunity, and preventing tumor occurrence. The nutritional requirements of butyric acid bacteria have a significant impact on their growth. The commonly used feeding nitrogen sources are peptone, yeast extract, soybean meal, soybean flour hydrolyzate, fish meal, etc. The method of bacterial preparation, the invention patent CN201110287802.1, 2011) discloses the method of adding 10-20% glucose (carbon source) and 5-10% soybean powder hydrolyzate (nitrogen source) to the fermentation process of butyric acid bacteria; comparative literature 2 (a method for producing butyric acid bacteria preparati...

Claims

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Application Information

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Patent Type & AuthorityApplications(China)
IPC IPC(8): C12N1/20C12N1/38C12R1/145
CPCC12N1/20C12N1/38
Inventor李盘欣王志夏会丽许文亮黄亚男史咏华彭丽王培英
Owner河南省南街村(集团)有限公司