A fully integrated nucleic acid detection microfluidic chip and its use method

A microfluidic chip and integrated nucleic acid technology, applied in chemical instruments and methods, biochemical equipment and methods, laboratory containers, etc., can solve problems such as false positives and aerosol pollution, and achieve smooth flow and simplified structure , the effect of saving the number of parts

Active Publication Date: 2019-11-26
BEIJING BAIKANGXIN BIOLOGICAL SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The difference between nucleic acid detection and biochemical and immunological detection is that aerosol pollution is easily generated during nucleic acid amplification, resulting in false positive results. Therefore, the entire microfluidic chip needs to be airtight.

Method used

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  • A fully integrated nucleic acid detection microfluidic chip and its use method
  • A fully integrated nucleic acid detection microfluidic chip and its use method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0046] A fully integrated microfluidic chip for nucleic acid detection, comprising a cover, a substrate, a negative, a sample box and a blister, the cover and the negative bond the two sides of the substrate, and the substrate is provided with a sample outlet Mouth, channel, sample box groove, at least one bubble cap groove and at least one reaction pool, the channel is respectively communicated with the sample box groove, each bubble cap groove and each reaction pool, and each reaction pool is connected to the sample outlet The sample box is arranged on the groove of the sample box, the groove of the sample box is connected with at least one reaction pool, the blister is arranged on the groove of the blister, reagents are placed in the blister, the Each groove of the bubble cap is respectively connected with the groove of the sample box and at least one reaction pool.

[0047] When in use, first place the sample in the sample box, make the sample pass through the groove of th...

Embodiment 2

[0059] On the basis of Example 1, a method for using a fully integrated nucleic acid detection microfluidic chip comprises the following steps:

[0060] Step S1, the user adds the sample into the sample box, so that the sample enters the groove of the sample box; squeezes the blister with an external instrument, adds reagents into the groove of the sample box, and obtains the processed sample;

[0061] Step S2, the external instrument pushes the processed sample through the pump interface, so that the processed sample passes through the adsorption pool, the magnetic bead capture pool and the amplification pool in sequence, and when passing through each reaction pool, use the external instrument to squeeze the blister according to the reaction requirements, Add corresponding reagents to the reaction cell to finally obtain the pre-detection solution;

[0062] Step S3, the pre-detection solution obtained in step S2 enters the detection instrument through the sample outlet for det...

Embodiment 3

[0075] On the basis of Embodiment 1 and Embodiment 2, this embodiment combines Figure 1-Figure 2 to illustrate the present invention.

[0076] like figure 1 , figure 2 As shown, a fully integrated nucleic acid detection microfluidic chip includes a cover sheet 1, a substrate 2, a bottom sheet 3, a sample box 4 and a blister 5. The cover sheet 1 and the bottom sheet 3 connect both sides of the substrate 2. The substrate 2 is provided with a sample outlet, a channel, a sample box groove 201, at least one blister groove 202 and at least one reaction pool, and the channel is respectively connected with the sample box groove 201, each blister groove The groove 202 is in communication with each reaction cell, and each reaction cell is connected with the sample outlet; the sample box 4 is arranged on the sample box groove 201, and the sample box groove 201 is connected with at least one reaction cell, and the blister 5 It is provided on the blister groove 202, and the blister 5 ...

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Abstract

The invention relates to a fully-integrated nucleic acid detection microfluidic chip and a use method thereof. The fully-integrated nucleic acid detection microfluidic chip comprises a cover plate, a substrate, a bottom plate, a sample box and bubble caps, wherein the cover plate and the bottom plate are respectively stuck to two sides of the substrate; the substrate is provided with a sample outlet, a channel, a sample box groove, at least one bubble cap groove and at least one reaction tank, wherein the channel is communicated with the sample box groove, all the bubble cap grooves and all the reaction tanks, and all the reaction tanks are respectively connected with the sample outlet; the sample box is arranged on the sample box groove; the sample box groove is connected with the at least one reaction tank; the bubble caps are arranged on the bubble cap grooves; the bubble caps are internally filled with a reagent; the bubble cap grooves are respectively connected with the sample box groove and the at least one reaction tank. The invention also provides the use method of the fully-integrated nucleic acid detection microfluidic chip. The fully-integrated nucleic acid detection microfluidic chip can be used for treating a complex sample in an integrated way, and is good in airtight property and simple in structure.

Description

technical field [0001] The present invention relates to the technical field of nucleic acid amplification detection, in particular to a fully integrated nucleic acid detection microfluidic chip and a method for using the same. Background technique [0002] The microfluidic chip is based on the micro-electromechanical processing technology, and a network is formed on the chip by the micro-pipeline, and the controllable micro-fluid runs through the whole system and completes various biological and chemical processes. In the early development of microfluidic chip technology, chip capillary electrophoresis was the mainstream technology, and the chip used was simple in structure and single in function; in recent years, microfluidic chips began to develop rapidly in the direction of functionalization and integration, such as nucleic acid amplification reactions, immune Important biological and chemical processes such as reactions and cell lysis have become new hotspots. With the ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12M1/34C12M1/00C12Q1/6844
CPCB01L3/5027B01L2200/12B01L2300/0803B01L2300/087B01L2300/0887C12Q1/6844C12Q2523/308C12Q2565/629
Inventor 张国豪李永峰
Owner BEIJING BAIKANGXIN BIOLOGICAL SCI & TECH
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