Pseudo-ginseng and spurious breed thereof identification method based on ultraviolet visible spectroscopy and chemical mode recognition

Abstract

The invention relates to a pseudo-ginseng and spurious breed thereof identification method based on ultraviolet visible spectroscopy and chemical mode recognition. The method specifically includes: collecting a certain number of pseudo-ginseng and spurious breed thereof, drying, crushing, screening with a 120-mesh sieve, and storing in a sealed plastic bottle; determining testing parameters of an ultraviolet visible spectroscopy instrument, and collecting ultraviolet visible spectra of samples; using a KS method to divide each traditional Chinese medicine, using 2 / 3 of the samples for training and 1 / 3 of the samples for predicting, and merging training data of all types into a total training set and predicting data into a total predicting set; determining PLS-DA factor number, building a PLS-DA model, and substituting the spectra of the samples in the predicting set into the PLS-DA model to acquire type of the samples in the predicting set. Spectral data are acquired through an ultraviolet visible spectrophotometer, so that detection is quick, environment-friendly and nondestructive; a PLS-DA method is adopted for modeling to build the model for detecting the pseudo-ginseng and the spurious breed thereof, so that accuracy is high. The identification method is suitable for identifying pseudo-ginseng and the spurious breed thereof.

Description

technical field [0001] The invention belongs to the technical field of traditional Chinese medicine detection, and in particular relates to a method for identifying Panax notoginseng and counterfeit products thereof based on ultraviolet-visible spectrum and chemical pattern recognition. Background technique [0002] Panax notoginseng, also known as Shanqi, Tianqi and Jinbuhuan, is a precious traditional Chinese medicine. It is the dried root of Panax notoginseng of the Araliaceae plant. It has the functions of stopping bleeding, dispersing blood stasis, reducing swelling and relieving pain, and improving memory. It is widely used in the treatment of cardiovascular and cerebrovascular system, nervous system, immune system and other diseases. Due to the shortage of Panax notoginseng resources in the market, the price keeps rising, and counterfeit products often appear driven by economic interests. Such as galangal, turmeric and curcuma. Not only has it caused confusion in th...

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Problems solved by technology

However, these methods have the disadvantages of heavy workload, expensive equipment and time-consuming

Molecular identification methods use modern molecular biology techniques to objectively reflect the differences in gene segments of species at the molecular level (Li Wenli, Xiao Bingyi, Luo Huiming, Nie Ping, Liu Li, Shu Biqiong, Ding Ye, Sun Hui, a method for identifying Panax notoginseng Specific primer pairs and methods, 2015, CN201510270247.X), the analysis results are reliable, but the operation is complicated, and there are criteria problems for judging species boundaries and intra-species variation ranges

[0004] Ultraviolet-visible spectroscopy has the advantages of cheap equipment, fast analysis speed, simple operation, and no pollution. It has been widely used in the identification of organic substances, but it has not been used in the identification of Panax notoginseng and its counterfeit products.

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