Micrococcus luteus strain and application thereof

A technology of Micrococcus luteus and strains, applied in the field of microbiology, can solve problems such as animal husbandry and vegetation damage, destruction of aquatic ecological environment, etc., and achieve the effects of alleviating harm, being environmentally friendly, and having good application prospects.

Active Publication Date: 2017-09-08
GUIZHOU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The water quality in polluted surface water bodies will gradually acidify, and when used to raise or water plants,

Method used

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  • Micrococcus luteus strain and application thereof
  • Micrococcus luteus strain and application thereof
  • Micrococcus luteus strain and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0035] The isolation of Micrococcus luteus GZU-Mi01 comprises the following steps:

[0036] 1. Isolation and screening of functional strain GZU-Mi01

[0037] 1) Sample collection

[0038] (1) The samples were collected from weathered coal gangue on a sulfur-containing coal gangue hill, and then the collected samples were put into plastic sample collection bags.

[0039] (2) Break up the collected samples.

[0040] (3) Sieve and collect 100-mesh samples.

[0041] (4) Put the samples in a refrigerator at 4°C for later use.

[0042] 2) Screening of functional strains

[0043] (1) Accurately weigh 1g of sample into a sterilized 250mL Erlenmeyer flask, and add 99mL of sterile water.

[0044] (2) Then shake for 30 minutes in a shaker at 170r / min and 37°C.

[0045] (3) For the control group, add 100mL sterile water to a 250mL Erlenmeyer flask.

[0046] (4) After half an hour, take the supernatant and gradually dilute to 10 -3 , 10 -4 , 10 -5 , 10 -6 , 10 -7 .

[0047] (5...

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Abstract

The invention discloses a micrococcus luteus strain and an application thereof. The micrococcus strain is morphologically characterized in that the appearance of the bacterial colony of the strain on an LB culture medium is golden yellow, in a concave and cone-frustum shape, opaque, even in edge, smooth and moist in surface, and shiny, and the size of the bacterial colony is approximately 2 millimeters. The strain is gram-positive tetracoccus. The micrococcus luteus strain is a strain which can efficiently decompose high-sulfur coal gangue and release phosphorus.

Description

technical field [0001] The invention belongs to the technical field of microbiology, specifically relates to a micrococcus luteus, and also relates to the use of the strain in dissociating and releasing phosphorus in high-sulfur coal gangue. Background technique [0002] In countries where coal is the main energy source, most coal resources are abundant, complete in variety, and widely distributed. Coal plays a pivotal role in economic development. In coal mining and processing, 20-25% of coal gangue will be produced. At present, there are billions of tons of coal gangue discarded in China alone. According to the level of sulfur content in coal, coal can be divided into low-sulfur coal and high-sulfur coal. The sulfur content in coal varies greatly, with the highest being 8% and the lowest being 0.12%. After the exposed coal gangue is piled up, some harmful substances are dissolved after being washed by rainwater, and flow into the soil, surface water body or underground ...

Claims

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Application Information

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IPC IPC(8): C12N1/20C12R1/265
CPCC12N1/205C12R2001/265
Inventor 谢承卫高弦钟艳李春钢王柳兴李夏夏姜雄
Owner GUIZHOU UNIV
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