Yogurt containing phosphatidylserine and preparation method thereof
A technology of phosphatidylserine and fatty acylserine, applied in the field of phosphatidylserine-containing yogurt and its preparation, can solve the problems of DHA instability, easy oxidation, and increased gastrointestinal burden
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[0014] The invention provides a method for preparing yogurt containing phosphatidylserine, the preparation method comprising:
[0015] (1) DHA algae oil, polysorbate 80 and water are mixed to obtain mixture A;
[0016] (2) Mix L-serine, phospholipase D and buffer solution, and heat to obtain mixture B;
[0017] (3) After mixing mixture A and mixture B, solvent extraction is performed to obtain phosphatidylserine rich in DHA;
[0018] (4) The phosphatidylserine obtained in step (3), fresh milk, thickener, emulsifier, twisted sweetener and lactic acid bacteria are mixed and then fermented to obtain yoghurt.
[0019] In a preferred embodiment of the present invention, in order to further improve the human body's absorption of DHA and PS in yogurt, relative to 100 parts by weight of water, the amount of DHA algae oil is 0.2-0.6 parts by weight, and the poly The consumption of sorbide 80 is 2-8 parts by weight, relative to the buffer solution of 100 parts by weight, the consumpti...
Embodiment 1
[0030] 0.2gDHA algae oil, 2g polysorbate 80 and 100g water are mixed to obtain mixture A; 10gL-serine, 0.5g phospholipase D and 100g buffer solution (buffer solution is composed of chloroform and ethanol, and the volume ratio of chloroform and ethanol 1:2) mixing and heating (the heating temperature is 40°C and the heating time is 4h) to obtain mixture B; mix mixture A and mixture B (mixture A and mixture B are mixed according to the volume ratio of 1:20) After extraction with a solvent (the solvent is obtained by mixing 20g ethanol, 10g ether and 100g water), the phosphatidylserine rich in DHA is obtained; Yogurt A1 is obtained by mixing 1 g of ester, 0.5 g of twisted sweetener and 2 g of lactic acid bacteria and then fermenting.
Embodiment 2
[0032] 0.6gDHA algae oil, 8g polysorbate 80 and 100g water are mixed to obtain mixture A; 20gL-serine, 5.5g phospholipase D and 100g buffer solution (buffer solution is made up of chloroform and ethanol, and the volume ratio of chloroform and ethanol 1:5) mixing and heating (the heating temperature is 60°C, and the heating time is 8h) to obtain mixture B; mix mixture A and mixture B (mixture A and mixture B are mixed according to the volume ratio of 1:40) After extraction with a solvent (the solvent is obtained by mixing 40g ethanol, 20g ether and 100g water), the phosphatidylserine rich in DHA is obtained; 2g, 0.7g of twisted sweet and 2.5g of lactic acid bacteria are mixed and fermented to obtain yogurt A2.
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