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Preparation method for phosphatidase D

A phospholipase and barley technology, applied in biochemical equipment and methods, enzymes, hydrolytic enzymes, etc., can solve the problem that barley roots cannot be effectively used

Inactive Publication Date: 2017-09-15
WUHU FOMAN BIOPHARMA CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] With respect to the above-mentioned prior art, the purpose of the present invention is to overcome the barley root in the prior art, and there is basically no situation that needs to be used in large quantities. Therefore, the barley root will often be treated as production waste, resulting in barley root The problem of not being effectively utilized, and then providing a method for preparing phospholipase D that can use barley root as a raw material to greatly reduce production costs

Method used

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preparation example Construction

[0013] The present invention provides a kind of preparation method of phospholipase D, wherein, described preparation method comprises:

[0014] 1) Extracting the barley root with water to obtain the extract M1;

[0015] 2) After centrifuging the extract M1 at least once, take the supernatant M2;

[0016] 3) adding ammonium sulfate solution to the supernatant M2, mixing and salting out, centrifuging, and removing the lower layer to obtain phospholipase D.

[0017] In the present invention, the barley root is leached with water first, and then the leached product is centrifuged at least once to remove the centrifuged supernatant, and then ammonium sulfate solution is added to the supernatant for salting out, so that the barley root is further extracted The phospholipase D is extracted, and then centrifuged again, and the centrifuged precipitate is taken to obtain a product containing phospholipase D.

[0018] The consumption of each material here can be selected according to ...

Embodiment 1

[0027] 1) Extract 10 parts by weight of barley root powder and 100 parts by weight of water at 30°C for 15 hours to obtain extract M1;

[0028] 2) Centrifuge the extract M1 at a rate of 1500r / min for 5min, then centrifuge at a rate of 3000r / min for 3min, and take the supernatant M2;

[0029] 3) Add 50 parts by weight of ammonium sulfate solution (concentration: 30% by weight) to the supernatant M2, mix and salt out for 3 hours, centrifuge at a speed of 3000 r / min for 8 minutes, remove the lower layer of sediment, and prepare an enzyme solution containing phospholipase D A1. (enzyme activity is 97.2U / g)

Embodiment 2

[0031] 1) Extract 10 parts by weight of barley root powder and 300 parts by weight of water at 40°C for 30 hours to obtain extract M1;

[0032] 2) Centrifuge the extract M1 at a rate of 3000r / min for 20min, then centrifuge at a rate of 5000r / min for 10min, and take the supernatant M2;

[0033] 3) Add 200 parts by weight of ammonium sulfate solution (concentration: 30% by weight) to the supernatant M2, mix and salt out for 6 hours, centrifuge at a rate of 6000 r / min for 15 minutes, remove the lower layer of sediment, and prepare an enzyme solution containing phospholipase D A2. (enzyme activity is 93.5U / g)

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Abstract

The invention discloses a preparation method for phosphatidase D. The preparation method comprises the following steps: 1) adding water into barley roots for extraction so as to prepare an extractive substance M1; 2) subjecting the extractive substance M1 to centrifugation at least once and collecting supernatant M2; and 3) adding an ammonium sulfate solution into the supernatant M2 for mixing and salting-out, carrying out centrifugation and collecting a precipitate at a lower layer so as to obtain phosphatidase D. According to such a technical scheme, water is added into the barley roots for extraction, a product of the extraction undergoes extraction at least once, then the supernatant obtained after centrifugation is collected, and the ammonium sulfate solution is added into the supernatant for salting out, so phosphatidase D in the barley roots is further extracted; and centrifugation is carried out again, and the precipitate obtained after centrifugation is collected, so a product containing phosphatidase D is obtained.

Description

technical field [0001] The invention relates to the field of production and preparation of phospholipase, in particular to a preparation method of phospholipase D. Background technique [0002] As a raw material for the preparation of phosphatidylserine, phospholipase D is widely used in production. As we all know, barley malt is often a large amount of raw material needed in brewing and other productions, and as the barley root that is bound to be produced in the production of barley malt, there is often basically no situation that requires a large amount of use. Therefore, barley root is often used. Dispose of as production waste. But barley root contains a lot of phospholipase D. [0003] Therefore, it is an urgent problem to be solved in the present invention to provide a method for preparing phospholipase D that can use barley root as raw material to obtain phospholipase D and greatly reduce the production cost. Contents of the invention [0004] With regard to the...

Claims

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Application Information

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IPC IPC(8): C12N9/16
CPCC12N9/16C12Y301/04004
Inventor 郑志强
Owner WUHU FOMAN BIOPHARMA CO LTD
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