Xinjiang wild apple seed germination-breaking method
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A seed and apple technology, applied in the field of apple seed germination, can solve the problems of seedlings without lateral roots, not easy to survive, easy to rot, etc., and achieve the effects of fast germination, reduced rot rate, and not easy to breed bacteria.
Inactive Publication Date: 2017-09-22
NORTHEAST FORESTRY UNIVERSITY
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[0005] The purpose of the present invention is to solve the problem that the existing wild apple seeds are perishable in the germination process after peeling, and the obtained seedlings have no lateral roots and are not easy to survive, and provide a method for accelerating germination of wild apple seeds in Xinjiang
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specific Embodiment approach 1
[0017] Specific embodiment one: the method for accelerating germination of Xinjiang wild apple seeds in this embodiment is implemented in the following steps:
[0018] 1. Soak Xinjiang wild apple seeds in distilled water for 12-24 hours, then rinse the seeds with distilled water, filter and obtain soaked Xinjiang wild apple seeds;
[0019] Two, adopt dissecting needle to peel off the seed coat of the Xinjiang wild apple seed after soaking, until exposing milky white cotyledon, put in water then, prevent dehydration, obtain the Xinjiang wild apple seed after peeling;
[0020] 3. Clean the vermiculite with distilled water, then cover it in a 15cm petri dish, add distilled water until the vermiculite is fully wet, place the peeled Xinjiang wild apple seeds evenly on the surface of the vermiculite, and place the petri dish with seeds Place them in a tissue culture room at 25°C for light culture, add distilled water every day during the culture period, turn the seeds over in the fi...
specific Embodiment approach 2
[0021] Embodiment 2: The difference between this embodiment and Embodiment 1 is that in step 1, Xinjiang wild apple seeds are soaked in distilled water for 16-20 hours. Other steps and parameters are the same as those in the first embodiment.
specific Embodiment approach 3
[0022] Specific embodiment three: the difference between this embodiment and specific embodiment one or two is that in step one, the soaked Xinjiang wild apple seeds are wrapped in moist gauze. Other steps and parameters are the same as those in Embodiment 1 or 2.
[0023] Since moist seeds are easier to peel, wrap wild apple seeds in moist gauze to prevent the seeds from drying out too quickly.
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Abstract
A Xinjiang wild apple seed germination-breaking method. The invention relates to a germination-breaking method of apple seeds, to solve the problem that in the prior art, peeled wild apple seeds are prone to decay in a germination process, and obtained seedlings have no lateral roots and are not easy to survive. The germination-breaking method includes: 1, putting Xinjiang wild apple seeds in distilled water for soaking for 12-24h, and washing the obtained apple seeds to make the seeds clean; 2, peeling coats of the soaked Xinjiang wild apple seeds off with a dissecting needle till milky cotyledons are exposed, and putting the obtained seeds in water to obtain peeled Xinjiang wild apple seeds; and 3, washing vermiculite with distilled water to make vermiculite clean, paving a 15 cm of a culture dish with vermiculite, adding distilled water to fully moisten vermiculite, uniformly putting the peeled Xinjiang wild apple seeds on the surface of vermiculite, and putting the culture dish filled with seeds in a tissue culture chamber at 25 DEG C for light culture. According to the method, vermiculite is taken as a moisture retention medium, so that the seeds are not prone to decay and can germinate at about 6 d. The transplanting survival rate is 100%, so that a large number of transplantable seedlings can be cultured.
Description
technical field [0001] The invention relates to a method for accelerating germination of apple seeds. Background technique [0002] Xinjiang wild apple (Malus sieversii) is an arbor of the genus Malus in the Rosaceae family. It belongs to the relict species of the Tertiary Period and is listed as an endangered second-class key protected plant in China. It is only distributed in the Tianshan Mountains of Central Asia, and only in Xinjiang in China. Yili and Tacheng areas. Studies have shown that Xinjiang wild apples are the ancestors of modern cultivated apples. It has formed many variations under long-term natural selection, including fruit shape, coloring, flavor, tree shape and other traits, and has strong environmental adaptability, and has excellent traits such as cold resistance, drought resistance and poor soil resistance. Therefore, wild apples in Xinjiang can provide a large number of seedlings and genetic resources with strong stress resistance for fruit tree prod...
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