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Rapid propagation method of skimmia japoniac

A technology of caprene and golden red, applied in the field of biology, can solve the problems of slow propagation of golden caprene and inability to maintain the excellent characteristics of the female parent, and achieve the effect of consistent plant sex, high breeding coefficient and obvious technical effect

Active Publication Date: 2017-09-22
SHANGHAI ACADEMY OF LANDSCAPE ARCHITECTURE SCI & PLANNING
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The object of the present invention is to provide a method for rapid propagation of genus genus. The rapid propagation method of genus genus should solve the problems in the prior art that genus genus propagation is slow, seed sowing, and cutting offspring cannot maintain the excellent characteristics of the female parent. technical problem

Method used

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  • Rapid propagation method of skimmia japoniac
  • Rapid propagation method of skimmia japoniac
  • Rapid propagation method of skimmia japoniac

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] Embodiment 1 surface sterilization

[0033] 1.1 Sampling

[0034] From March to May, take samples in sunny afternoons, remove the branches and leaves, and cut the stems into 2cm long sections, each with an axillary or terminal bud. Rinse with running water for 1h to 2h, and soak in 60% to 80% ethanol for 30s to 60s on the ultra-clean workbench, then soak in different sterilizing agents for different times, and finally rinse with sterile water for 5 to 6 times. Screen the best sterilizing agent and sterilization time.

[0035] 1.2 Effects of different sterilization times on the survival rate of explants

[0036] Set 5 different sterilization times of 0.15% (mass to volume ratio, the same below) mercuric chloride for 6 minutes, 8 minutes, 10 minutes, 12 minutes and 15 minutes, compare the effect on the survival rate of explants, and repeat 3 times. Survival rate=survival number / inoculation number×100%.

[0037] The results showed that when the concentration of 0.15% m...

Embodiment 2

[0046] Embodiment 2 first-generation induction

[0047] MS was used as the basal medium in the stages of primary culture, proliferation culture and rooting culture, and different types of hormones and their different concentrations were added. See Table 4 and Table 5 for details.

[0048]In the primary induction medium, ZT was set at 6 concentrations of 0.01, 0.05, 0.1, 0.2, 0.5, and 1.0 mg / L; IBA was set at 5 concentration gradients of 0.01, 0.02, 0.04, 0.05, and 0.1 mg / L; 3 There are 8 concentrations of 0.5, 1, 2, 4, 5, 6, 8, 10, and 12 mg / L.

[0049] After the initial culture of sterile explants for 30 days, the results showed that when the concentration of ZT was 0.05mg / L~0.5mg / L, the explants could germinate and grow, and when the concentration was 0.2mg / L, the germination of explants was induced When the concentration is 0.5 mg / L and above, although the induction rate reaches 65%, there is differentiation phenomenon in the later stage of culture, which is not conducive ...

Embodiment 3

[0058] Embodiment 3 Proliferation culture

[0059] In the proliferation stage, ZT was set at 5 concentrations of 0.1, 0.5, 1.0, 2.0, and 3.0 mg / L; KT was set at 0.01, 0.05, 0.1, 0.5, 1, and 2 mg / L at 6 concentrations; IBA was set at 0.01, 0.02, 6 concentrations of 0.05, 0.1, 0.2, 0.3mg / L; add GA at the same time 3 5mg / L.

[0060] The test-tube plantlets induced on the first-generation induction medium, when they grow up gradually, and a certain amount of test-tube plantlets are obtained, select the test-tube plantlets with a height of 0.5cm and light green color, and transfer them to the proliferation medium in the same batch, and select the suitable proliferation medium . The results showed that when the concentration of ZT was 0.5-2mg / L, the proliferation coefficient reached more than 2, and when the concentration was 1mg / L, the proliferation coefficient reached the highest value of 2.4, which was significantly different from other treatments (Table 6).

[0061] Table 6 E...

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Abstract

The invention provides a rapid propagation method of skimmia japoniac. Stems with axillary buds are adopted as explants, and disposed by sterilization treatment and then isolated culture. An initial culture medium is 0.05-0.5 mg / L of MS and ZT, 0.02-0.05 mg / L of IBA and 2-10 mg / L of GA3 , wherein the highest inductivity rate reaches 85 %; an enrichment medium is 0.5-2 mg / L of MS and ZT, 0.05-1 mg / L of KT, and 0.02-0.2 mg / L of IBA , wherein the whole cultivation period lasts for 30 days, the highest increase rate is 3.6, and the height of test-tube plantlets is 2.0 cm; an root medium is 1.0-2.0 mg / L of 1 / 2 MS and IBA, wherein the highest rate of rooting is 90 %; finally by transplanting the test-tube plantlets into a combined matrix compounded by peat, vermiculite, perlite and organic fertilizer, wherein the rate of survival can reach 80% or above. A propagation system formed by the rapid propagation method of skimmia japoniac has the advantages of being uniform in plant character of propagated offspring, high in propagation coefficient, free of seasonal climate, applicable to anniversary production, and capable of meeting demand of seeds for production use anytime.

Description

technical field [0001] The invention belongs to the field of biology, and relates to a method for plant propagation, in particular to a method for rapid propagation of taro. Background technique [0002] The plant of the genus Skimmia Thunb is an evergreen flowering shrub of Rutaceae. There are 7 or 8 species known in the world. They are distributed from the Himalayas to Japan. There are 3 or 4 species in my country. They are produced from southwest to south, wild in forests and valleys , roadside, etc. It likes a warm climate and a place with sufficient light, and it is also slightly shade-tolerant. It likes moist, fertile loam, and is not cold-tolerant. It is often planted on the edge of the forest in the garden, and can also be used as a bonsai. In addition, Yinyu is also a traditional Chinese medicine with analgesic and anti-inflammatory effects. Relevant scholars have done a lot of research on the composition, pharmacology, and clinical application of Yinyu. At present...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
CPCA01H4/001A01H4/008
Inventor 吕秀立方腾郭小芳付瑞王铖边黎明
Owner SHANGHAI ACADEMY OF LANDSCAPE ARCHITECTURE SCI & PLANNING
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