Indoor artificial breeding propagation method for yellow sugarcane aphids
A sugarcane and artificial technology, applied in the field of artificial breeding of sugarcane yellow aphid indoors, can solve the problems of poor uniformity, influence on growth and reproduction, and inability to ensure sufficient experimental materials, etc., achieve low cost, easy operation, and avoid the spread of crop pathogens and the prevalence of corresponding diseases
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Embodiment 1
[0022] Embodiment 1, a kind of sugarcane yellow aphid indoor artificial breeding method comprises the following steps:
[0023] Funong 41 sugarcane plants that grow robustly and are free from diseases and insect pests are taken from the field, cut into single-bud stems, and full-grown buds are selected, treated in a constant temperature circulating hot water bath at 52°C for 30 minutes, and placed at 37°C to accelerate germination. The height of the buds should not be lower than 5 cm, take the leaves, refer to the sugarcane mosaic virus and sorghum mosaic virus described in the literature published in "Annals of Applied Biology" (Luo et al., 2016) and "Virus Genes" (Gao et al., 2012) and the RT-PCR detection method of sugarcane yellow leaf virus. After confirming that no virus is carried, the buds are taken, and the buds are sterilized with 75% ethanol for 0~30 s, and then sterilized with 0.1% HgCl 2 Sterilize for 15 minutes, peel off 3-5 leaf sheaths, cut off the shoot tips w...
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