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CD44-shRNA/PEG-MZF-NPs compound and preparation method thereof

A PEG-MZF-NPS, cd44-shRNA technology, applied in the fields of medicine and genetic engineering, to achieve the effect of realizing the integration of targeted diagnosis and treatment, and good heating and constant temperature capability

Inactive Publication Date: 2017-09-22
JIANGSU TAIZHOU PEOPLES HOSPITAL
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0008] In order to solve the existing problems of gene therapy, the present invention provides a PEG-modified Mn 0.6 Zn 0.4 Fe 2 o 4 Nanoparticles (PEG-MZF-NPs) as a carrier CD44-shRNA anti-tumor gene complex and its preparation method, the complex can organically combine CD44-shRNA gene therapy and magnetic fluid hyperthermia under the action of an external magnetic field Combining and complementing each other's advantages, targeted therapy for tumors effectively solves the problems of existing technologies

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  • CD44-shRNA/PEG-MZF-NPs compound and preparation method thereof

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Embodiment

[0037] 1.1 PEG-MZF-NPS heating experiment in alternating magnetic field

[0038] Prepare PEG-MZF-NPS magnetic fluid with concentrations of 20μg / ml, 40μg / ml, 60μg / ml, and 80μg / ml, respectively, take 500μl and place them in a flat-bottomed test tube, and insert the thermometer directly into the test tube (avoid touching the tube wall) , Start the alternating magnetic field, set the parameters to 235KHZ, 4KW, 35A, the diameter of the magnetic induction coil is 3CM, the bottom of the test tube is about 0.5cm away from the magnetic induction coil, and record the temperature every 5 minutes. Use time as the abscissa and temperature as the ordinate, draw PEG -The heating curve of MZF-NPS magnetic nano material. See the heating characteristics of PEG-MZF-NPS in alternating magnetic field figure 1 , 60μg / ml magnetic fluid can be heated to about 43°C within 20min and remain stable, which can be used for tumor hyperthermia.

[0039] 1.2 In Vitro Magnetic Resonance Imaging (MRI) Study of PEG-...

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Abstract

The invention belongs to the technical field of medicine and genetic engineering, and particularly relates to a CD44-shRNA / PEG-MZF-NPs compound and a preparation method and application of the CD44-shRNA / PEG-MZF-NPs compound. The compound takes a PEG-MZF-NPs magnetic nanoparticle as a carrier to load a plasmid CD44-shRNA. The preparation method comprises the following steps: respectively using a serum-free medium to dilute the PEG-MZF-NPs and the plasmid CD44-shRNA, placing at the room temperature for more than 5 minutes, mixing the PEG-MZF-NPs with the plasmid CD44-shRNA evenly, and placing at the room temperature for more than 30 minutes, wherein the reactant is the CD44-shRNA / PEG-MZF-NPs compound. The invention further discloses the application of the compound to preparing a medicine for treating the ovarian cancer. The experiment shows that the compound has better effects of inhibiting proliferation and inducing apoptosis of ovarian carcinoma cells under the action of an additional alternating magnetic field, and the effects are obviously better than those of the single magnetofluid thermal therapy.

Description

Technical field [0001] The invention belongs to the technical field of medicine and genetic engineering, and specifically relates to a CD44-shRNA / PEG-MZF-NPs complex and a preparation method and application thereof. Background technique [0002] Ovarian cancer is one of the most common tumors in the female reproductive system, with a high degree of malignancy, and the mortality rate is the first among gynecological tumors. Because the symptoms of ovarian cancer are often not obvious in the early stage, and it is not easy to detect it early, more than 70% of patients are in the middle and late stage when they see a doctor, and the 5-year survival rate is only 25%. At present, there is no specific and effective method or means for the early diagnosis of ovarian cancer. At present, the treatment of ovarian cancer is mainly supplemented with chemotherapy after surgery, but conventional chemotherapy has relatively large side effects, and the recurrence rate and metastasis rate after ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K48/00A61K47/60A61K47/69A61K41/00A61K9/127A61K9/00A61P35/00
Inventor 林梅郭婷
Owner JIANGSU TAIZHOU PEOPLES HOSPITAL