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Application of hsa_circRNA_104907 to diagnosis, treatment and prognosis of Down's syndrome

A technology of Down syndrome and RNA primers, which is applied in the direction of DNA/RNA fragments, recombinant DNA technology, microbial measurement/inspection, etc., can solve the problem of low content of nucleated cells in the fetus, the inability to meet the requirements of analysis and diagnosis, and the limitations of clinical applications And other issues

Active Publication Date: 2017-09-22
中国人民解放军第一八一医院
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the biggest problem with this method is that the content of fetal nucleated cells in maternal blood is very small, which cannot meet the requirements of analysis and diagnosis, and ultimately limits the clinical application.

Method used

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  • Application of hsa_circRNA_104907 to diagnosis, treatment and prognosis of Down's syndrome
  • Application of hsa_circRNA_104907 to diagnosis, treatment and prognosis of Down's syndrome
  • Application of hsa_circRNA_104907 to diagnosis, treatment and prognosis of Down's syndrome

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] In the process of research on Down syndrome, a circular RNA was discovered, its sequence is:

[0021] GTGTGTTCCAGAGAACAAGCCTTCAGACATTTGCTATATTGACGCTGAGCTGTCAGGGGACTCAGGCTTATGAGGAGGTATTGTTACACAAAACAGTGTCTAGTGAAGACGACAAGAAAGAGGGGAAAGGATCGGAAAAAGAAGCTAAAATACTATAGAAAACCATGAGATCTATTCGATCTTTTGCTAATGATGATCGCCATGTTATGGTGAAACATTCAACAATCTATCCATCTCCGGAGGAACTTGAAGCTGTTCAGAATATGGTATCTACTGTTGAATGTGCTCTTAAACATGTCTCAGATTGGTTGGATGAAACAAATAAAGGCACAAAAACAGAGGGTGAGACAGAAGTGAAGAAAGATGAGGCCGGAGAAAACTATTCCAAGGATCAAGGTGGTCGGACATTGTGTGGTGTAATGAGGATTGGCCTGGTTGCAAAAGGCTTGCTGATTAAAGATGATATGGACTTGGAGCTGGTTTTAATGTGCAAAGACAAACCCACAGAGACCCTGTTAAATACAGTCAAAGATAATCTTCCTATTCAGATTCAGAAACTCACAGAAGAGAAATATCAAGTGGAACAATGTGTAAATGAGGCATCTATTATAATTCGGAATACAAAAGAGCCCACGCTAACTTTGAAGGTGATACTTACCTCACCTCTAATTAGGGACGAATTGGAGAAGAAGGATGGAG (SEQ ID NO: 1), named hsa_circRNA_104907.

Embodiment 2

[0022] Example 2 Sample and processing

[0023] Twelve fetal cord blood specimens were obtained from the Central Laboratory of the Clinical Medicine Research Center of Shenzhen People’s Hospital, including 6 cord blood specimens (2 males 4 females) diagnosed as standard Down syndrome fetuses by G-band karyotype analysis ) And 6 cases of normal fetal umbilical cord blood (2 males and 4 females), gestational ages were 18-22 weeks; 12 peripheral blood specimens of children were obtained from the Guangxi Key Laboratory of Metabolic Disease Research, the 181st Army Hospital of the Chinese People’s Liberation Army, Guilin Obtained, including 6 peripheral blood specimens of children with Down syndrome (2 males and 4 females) and 6 peripheral blood specimens of healthy children (2 males and 4 females), all aged 0-15 years old. This study has been approved by the Ethics Committee of Shenzhen People's Hospital and the 181st Army Hospital of the People's Liberation Army of Guilin, and the p...

Embodiment 3

[0043] Example 3 Experimental results

[0044] Using β-actin as an internal reference, RT-PCR technology was used to verify the above circRNA in peripheral blood. The PCR primer sequence is shown in Table 1:

[0045] Table 1 RT-PCR primer list

[0046]

[0047] Use the 2-ΔΔCt method to calculate the difference multiples, and the results are shown in Table 2.

[0048] Table 2 circRNA verification results

[0049]

[0050] The results show that compared with healthy people, the expression of hsa_circRNA_104907 in Down syndrome patients will increase significantly, and there is a significant positive correlation between the two.

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Abstract

The invention discloses new circular RNA: hsa_circRNA_104907 to diagnosis, wherein the sequence is as shown in SEQ ID NO:1; meanwhile, the invention further discloses a RT-PCR primer for detecting the circular RNA. Relative to normal people, the expression of the circular RNA in the body of a patient suffering from Down's syndrome is remarkably up-regulated, and the expression up-regulation has statistically significant correlation after statistics. Therefore, the circular RNA has potential of serving as a Down's syndrome related biomarker.

Description

Technical field [0001] The present invention belongs to the field of biotechnology, and particularly relates to a new cyclic hsa_circRNA_104907 and its application. Background technique [0002] Down syndrome (Down Syndrome, DS), 21-trisomy syndrome, also known as congenital ignorance or Down syndrome, is a birth defect disease caused by an abnormality of chromosome 21. It is a birth defect disease One of the most common genetic diseases of chromosomal aneuploidy. Because the pathogenesis of Down syndrome is still unclear, there is still no effective treatment for the disease. Birth intervention is currently an important means to reduce birth defects, and birth intervention mainly depends on prenatal diagnosis and prenatal screening. Prenatal screening is mainly used in the first trimester (7-12 weeks) and the second trimester (15-20 weeks) to detect certain indicators of pregnant women, including maternal age screening, ultrasound screening, fetal maternal serology screening, a...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/68C12N15/11
CPCC12Q1/6883C12Q2600/118C12Q2600/158C12Q2600/178
Inventor 眭维国戴勇常燕薛雯欧明林
Owner 中国人民解放军第一八一医院
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