Lactic acid bacterium having purine body uptake ability, and use thereof

A technology of lactic acid bacteria and purine, applied in the field of lactic acid bacteria, to achieve the effect of reducing the level of serum uric acid

Inactive Publication Date: 2017-09-26
MEIJI CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, few reports are available on human test results, and an effective method for obtaining lactic acid bacteria with the effect of reducing serum uric acid levels is unknown

Method used

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  • Lactic acid bacterium having purine body uptake ability, and use thereof
  • Lactic acid bacterium having purine body uptake ability, and use thereof
  • Lactic acid bacterium having purine body uptake ability, and use thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0086] [Example 1] Test for evaluating purine uptake ability

[0087] In this example, the purine uptake ability of the Lactobacillus gasseri OLL2959 strain was evaluated using purine labeled with a radioisotope (RI).

[0088] Lactobacillus gasseri OLL2959 strain was deposited on March 31, 2006 (original deposit date) under the accession number NITE P-224 at the National Institute of Technology Evaluation, Patent Microorganism Deposit Center (NPMD) (#122, 2-5-8 Kazusakamatari, Kisarazu -shi, Chiba 292-0818, Japan), and was subsequently transferred to a deposit under the Budapest Treaty (International Deposit) on November 21, 2007 under the changed accession number NITEBP-224 from the original accession number. Lactobacillus gasseri OLL2959 strain was inoculated into MRS medium (Lactobacillus MRS Broth, Difco Co., Ltd.), and cultured at 37° C. for 16 to 20 hours, the resulting culture (4 to 7×10 8 cfu / ml) are used below.

[0089] To 0.1 mL of minimal medium (DM medium; Table ...

Embodiment 2

[0097] [Example 2] Test for evaluating proliferative ability in the presence of purine

[0098] In this example, the Bacillus gasseri OLL2959 strain was cultured in the presence of purine, and its proliferative ability in the presence of purine was evaluated.

[0099] To 1 mL of DM medium (Table 1), adenosine monophosphate (AMP), adenosine, or adenine was added as purine to a final concentration of 400 μM, and then the culture of Lactobacillus gasseri OLL2959 strain prepared in Example 1 was With 4wt% (0.04mL:1.6 to 2.8x 10 7 cfu) and cultured anaerobically at 37°C. After 0 hour, 4 hours and 6 hours from the start of this culture, the turbidity (absorbance at 650 nm) of the medium was measured. As a control, the Lactobacillus gasseri OLL2959 strain was cultured in the same manner except that purine was not added to the minimal medium, and the turbidity of the medium was measured. The results are shown in figure 2 middle.

[0100] The results showed that in the presence o...

Embodiment 3

[0101] [Example 3] Comparative test of adenine uptake ability and proliferative ability in the presence of adenine

[0102] In this example, the Lactobacillus gasseri OLL2959 strain and other Lactobacillus gasseri strains were cultured in the presence of adenine, and the adenine uptake ability and proliferation ability in the presence of adenine of the respective strains were compared.

[0103] Lactobacillus gasseri P14054ME001 strain and P14054ME002 strain were used as other Lactobacillus gasseri strains. When culturing Lactobacillus gasseri P14054ME001 strain and P14054ME002 strain in purine-free MRS medium (Lactobacillus MRS broth, Difco, Co., Ltd.) for 20 hours, the proliferation ability of each strain was comparable to that of milk gasseri Bacillus OLL2959 strain was comparable (Table 2).

[0104] [Table 2]

[0105]

[0106] The evaluation test for adenine uptake ability was performed as described in Example 1, except that only adenine ( 14 C-adenine) as a radioisot...

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Abstract

Provided is a method for obtaining a lactic acid bacterium having a property of reducing a serum uric acid level. The present invention relates to: a method for screening for a lactic acid bacterium, which comprises determining the amount of a purine body incorporated in each of lactic acid bacteria in a culture medium containing the purine body and then screening for a lactic acid bacterium having a property of trapping the purine body among the lactic acid bacteria employing the amount as a measure; and a purine body trapping agent for reducing a serum uric acid level, which comprises a lactic acid bacterium obtained by the method.

Description

technical field [0001] The present invention relates to lactic acid bacteria with purine uptake ability and use thereof. Background technique [0002] In Japan in recent years, the number of patients suffering from gout or individuals with hyperuricemia has increased every year along with changes in diet. Hyperuricemia includes decreased excretion of uric acid or excessive uric acid production, and increased serum uric acid levels often induce gout, which develops into acute arthritis with severe pain. Currently in Japan, the number of patients suffering from gout is estimated to be one million, and the number of individuals with hyperuricemia is estimated to be ten million. Currently, hyperuricemia is mainly prevented or treated by controlling serum uric acid levels through a combination of diet therapy, exercise therapy, and drug therapy. In dietary therapy, calorie restriction is used to reduce the intake of dietary purines that eventually degrade to form uric acid. Ho...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/00A23L33/10A61K35/747A61P3/00C12N1/20
CPCA61K35/747C12N1/00C12N1/20
Inventor 山田成臣狩野宏岩本千鹤大柴幸男坪井洋浅见幸夫伊藤裕之
Owner MEIJI CO LTD
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