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Inducing method for rhododendron delavayi 'cosmopolitan' somatic embryo

A somatic embryo and king butterfly technology, applied in the field of ornamental plant biology, can solve the problems of lack of genetic transformation system

Pending Publication Date: 2017-10-13
SHIJIAZHUANG ACADEMY OF AGRI & FORESTRY SCI +1
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

At present, the research on Alpine Rhododendron 'Kingbuta' mainly focuses on the cultivation technology, physiological and biochemical aspects, and little research on molecular biological methods is due to the lack of the establishment of its genetic transformation system. During the establishment of the genetic transformation system, Plant regeneration through induction of somatic embryos is a bottleneck in the smooth development of research

Method used

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  • Inducing method for rhododendron delavayi 'cosmopolitan' somatic embryo
  • Inducing method for rhododendron delavayi 'cosmopolitan' somatic embryo
  • Inducing method for rhododendron delavayi 'cosmopolitan' somatic embryo

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Embodiment 1

[0034] Example 1. Using tissue culture method to induce alpine Rhododendron 'Pink Butterfly' leaves to undergo dedifferentiation to produce somatic embryos

[0035] Step 1: Harvesting the explants

[0036] Choose plants with good growth potential, cut off the young leaves at the top, and prepare for disinfection.

[0037] Step 2: Disinfection treatment First, brush with a toothbrush dipped in detergent diluent, then rinse with tap water for 30 minutes, treat with 75% alcohol on the ultra-clean workbench for 30S, rinse with sterile water 4-5 times, 0.1% HgCl 2 Treat for 2-3 minutes, then rinse with sterile water 4-5 times.

[0038] Step 3: Media preparation:

[0039] 1 liter of WPM minimal medium contains macroelements: NH 4 NO 3 400mg; (NH 4 ) 2 SO 4 132 mg; MgSO 4 .7H 2 O370mg; KH 2 PO 4 170mg; KNO 3 400 mg; K 2 SO 4 900mg; calcium salt: CaCl 2 96mg; Ca(NO 3 ) 2 4H 2 O 556mg; trace elements: H 3 BO 3 6.2 mg; MnSO 4 .H 2 O 22.3 mg; Na 2 MoO 4 2H ...

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Abstract

The invention belongs to the technical field of ornamental plant biology, and relates to a generating method for a rhododendron delavayi 'cosmopolitan' somatic embryo. The method for inducing generation of the somatic embryo by means of leaves of rhododendron delavayi 'cosmopolitan' mainly comprises the steps that tender leaves are disinfected and then inoculated onto a culture medium containing wpm, 0.2 mg / L of TDZ, 2 mg / L of 2ip, 0.25 mg / L of NAA, sucrose and agar, and after dark culture is conducted, light culture is conducted. According to the inducing method for the ornamental plant rhododendron delavayi 'cosmopolitan' somatic embryo, a foundation is laid for research on reestablishment of a rhododendron delavayi genetic transformation system, protoplast culture, excellent clone propagation genetic engineering and / or mutant screening and the like.

Description

technical field [0001] The invention belongs to the field of ornamental plant biotechnology, and specifically discloses a method for inducing somatic embryos by using the leaves of the alpine rhododendron 'Kingbutterfly'. Background technique [0002] The variety 'Cosmopolitan' (Rhododendron hybrides) is a hybrid cultivar of the Rhododendron family Rhododendron. It is an evergreen shrub and small tree with pink flowers and faded to pinkish-white edges, with a large brown red spot. Dense, stretching plant type, bright green leaves, cold-resistant -23 ℃ without falling leaves, strong disease resistance, with high ornamental value. At present, the research on Alpine Rhododendron 'Kingbuta' mainly focuses on the cultivation technology, physiological and biochemical aspects, and little research on molecular biological methods is due to the lack of the establishment of its genetic transformation system. During the establishment of the genetic transformation system, Achieving plan...

Claims

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Application Information

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IPC IPC(8): A01H4/00
CPCA01H4/00A01H4/001
Inventor 蒋淑磊李志斌白霄霞李国松李振勤边光亚刘伟李坤张骁骁刘星辰
Owner SHIJIAZHUANG ACADEMY OF AGRI & FORESTRY SCI
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