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Application of medicine composition containing black tea extract to sepsis treatment

A black tea extract and sepsis technology, applied in the application field of pharmaceutical compositions in the treatment of sepsis, can solve the problems of not being aware of the use of sepsis, and achieve the effects of improving survival rate and good inhibitory effect

Active Publication Date: 2017-10-17
INST OF RADIATION MEDICINE CHINESE ACADEMY OF MEDICAL SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In recent years, the research on black tea has been increasing, mainly focusing on its blood fat lowering, anti-oxidation, blood sugar lowering, sterilization, anti-radiation and other aspects, but did not realize its use in the treatment of sepsis

Method used

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  • Application of medicine composition containing black tea extract to sepsis treatment
  • Application of medicine composition containing black tea extract to sepsis treatment
  • Application of medicine composition containing black tea extract to sepsis treatment

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] Embodiment 1 Preparation of black tea water extract

[0022] Take 100g of Anhua Fuzhuan tea (purchased from Hunan Meishanya Anhua Black Tea R&D Center, the place of origin is Hunan Anhua) to remove dust and purify, crush and pass through a 60-mesh sieve, black tea: water = 1:10 (weight ratio), add distilled water Extract in a water bath at 80°C for 30 minutes to form a dark tea extract extract;

[0023] (2), filter:

[0024] The black tea extract extract is firstly filtered with gauze to obtain a coarse filtrate, which is repeatedly extracted and filtered for 3 times, and the coarse filtrate is combined;

[0025] (3), static:

[0026] Concentrate the crude filtrate by distillation under reduced pressure at 60°C with a rotary evaporator to 1 / 3 of the original volume, and then place it in a refrigerator at 4°C for 12 hours;

[0027] (4), fine filter:

[0028] Take the supernatant, and centrifuge through a centrifuge to remove the precipitate and suspended matter, and ...

Embodiment 2

[0034] Example 2 Preparation of black tea polysaccharide extract

[0035] (1), extraction

[0036] Take 100g of Anhua Fuzhuan tea (purchased from Hunan Meishanya Anhua Black Tea R&D Center, the place of origin is Hunan Anhua) to remove dust and purify, crush and pass through a 60-mesh sieve, black tea: water = 1:10 (weight ratio), add distilled water Extract in a water bath at 80°C for 30 minutes to form a dark tea extract extract;

[0037] (2), filter:

[0038] The black tea extract extract is firstly filtered with gauze to obtain a coarse filtrate, which is repeatedly extracted and filtered for 3 times, and the coarse filtrate is combined;

[0039] (3), static:

[0040] Concentrate the crude filtrate by distillation under reduced pressure at 60°C with a rotary evaporator to 1 / 3 of the original volume, and then place it in a refrigerator at 4°C for 12 hours;

[0041] (4), fine filter:

[0042] Take the supernatant, and centrifuge through a centrifuge to remove the precip...

Embodiment 3

[0050] Embodiment 3 Animal experiments

[0051]1. Therapeutic effect of black tea extract on LPS-induced sepsis in mice

[0052] (1), drug black tea extract (respectively prepared by embodiment 1, embodiment 2) dosage (calculated by black tea extract) is: black tea water extract 500mg / kg, black tea polysaccharide extract 500mg / kg kg.

[0053] (2) ICR male mice raised in an animal SPF grade barrier system, 6-7 weeks old, with a body weight of 20±2g. They were placed in dry and clean plastic cages, fed with standard experimental animal feed, and had free access to water and food.

[0054] (3) Experimental method Animals were randomly divided into groups according to body weight, 10 animals in each group. The experiment set up blank control group, LPS control group, dark tea water extract administration group and black tea polysaccharide administration group.

[0055] Three days before the intraperitoneal injection of 40 mg / kg LPS, the black tea water extract administration g...

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Abstract

The invention provides application of a black tea extract to sepsis treatment. The application of the black tea extract to the sepsis treatment is characterized in that the black tea extract is a black tea water extract or a black tea polysaccharide extract; a preparation method of the black tea polysaccharide extract comprises the following steps: crushing and sieving black tea, adding distilled water for extraction, filtering, taking a filtrate, performing reduced-pressure distillation and concentration, finely filtering, extracting the filtrate by using an organic solvent, taking a water phase, performing reduced-pressure distillation and concentration, freezing and drying to obtain the black tea polysaccharide extract. The invention discovers that the black tea extract can be used for the sepsis treatment and can greatly improve the survival rate of various factor-induced sepsis mice; moreover, the invention further discovers that black tea polysaccharide has a better inhibiting effect on induced macrophage programmed necrosis on a cell level. Therefore, the invention provides a reliable technical scheme that the black tea extract is applied to the sepsis treatment field and gives a realistic possibility that the black tea extract is taken as a medicine to be developed and pushed into the pharmaceutical market.

Description

technical field [0001] The invention belongs to the field of modern traditional Chinese medicine, and in particular relates to the application of a pharmaceutical composition containing black tea extract in treating sepsis. Background technique [0002] Sepsis is one of the thorny problems facing clinical practice, and the septic shock and multiple organ failure syndrome induced by it have been the main cause of death in surgical intensive care units (ICU) patients. Currently, 30%-45% of patients with sepsis still die due to failure to control their condition in time, and the incidence of sepsis continues to increase. Although good progress has been made in the treatment of critically ill patients due to the continuous development and updating of antibiotics, sepsis is still one of the persistent clinical problems. Sepsis is a systemic inflammatory response syndrome caused by infection or highly suspicious infection focus, and is a common complication after severe trauma, b...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/82A61K31/715A61P31/04
CPCA61K31/715A61K36/82
Inventor 龙伟牟感恩张广慧周则卫
Owner INST OF RADIATION MEDICINE CHINESE ACADEMY OF MEDICAL SCI
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