SNP marker associated with curative effect of novel adjuvant chemotherapy of breast cancer and application of SNP marker

A breast cancer and marker technology, applied in the field of SNP markers, can solve the problems of low pCR rate, NACT insensitivity, delayed local treatment, etc., to improve sensitivity and specificity, facilitate detection, improve pathological complete remission rate and Effects on long-term survival

Active Publication Date: 2017-10-17
AFFILIATED HUSN HOSPITAL OF FUDAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] The clinical application of NACT has a history of more than 30 years. Although great progress has been made in recent years, problems and deficiencies that need to be solved have also been discovered with the deepening of research: about 20% of breast cancer patients are not

Method used

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  • SNP marker associated with curative effect of novel adjuvant chemotherapy of breast cancer and application of SNP marker
  • SNP marker associated with curative effect of novel adjuvant chemotherapy of breast cancer and application of SNP marker
  • SNP marker associated with curative effect of novel adjuvant chemotherapy of breast cancer and application of SNP marker

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0048] This embodiment is the collection of samples and the arrangement of sample data.

[0049] From September 2015 to November 2016, the inventor collected a large number of blood samples from breast cancer chemotherapy patients at Huashan Hospital Affiliated to Fudan University. After sorting out the sample data, the inventor selected 90 samples that met the following criteria: (1) Females aged 18-70 years; (2) Breast cancer patients confirmed by pathological biopsy or cytology; (3) Measurable tumor lesions confirmed by mammography or B-ultrasound; (4) Accepted 4 cycles of neoadjuvant chemotherapy with cyclophosphamide (C) + epirubicin (E) + paclitaxel or docetaxel (T); (5) left / right mastectomy after neoadjuvant chemotherapy; (6) ) General physical condition score PS 0-2; (7) No major organ dysfunction, blood routine, liver and kidney function and heart function are basically normal; (8) Complete follow-up information can be obtained; Demographic and clinical data, etc. ...

Embodiment 2

[0051] This embodiment is the peripheral blood DNA extraction, genotype detection and result analysis.

[0052] Among the 90 eligible breast cancer patients in the above-mentioned embodiment, their disease state and physiological state were balanced and comparable, and there were statistical differences.

[0053] The specific steps are:

[0054] 1) Extract 200 μl fresh blood, add 20ul Proteinase K solution, and mix well;

[0055] 2) Add 200 μl buffer GB, mix thoroughly by inversion, place at 70°C for 10 minutes, and briefly centrifuge to remove water droplets on the inner wall of the tube cap;

[0056] 3) Add 200 μl of absolute ethanol, shake and mix well for 15 sec, and briefly centrifuge to remove water droplets on the inner wall of the tube cap;

[0057] 4) Add the solution and flocculent precipitate obtained in the previous step into an adsorption column CB3 (the adsorption column is placed in a collection tube), centrifuge at 12000rpm (~13400×g) for 30sec, pour off the ...

Embodiment 3

[0067] This example is the production of a kit for detecting or predicting the curative effect of paclitaxel-based neoadjuvant chemotherapy for breast cancer.

[0068] The production and operation process of the SNP kit is based on the Sequenom Mass ARRAY genotyping platform detection technology. The kit contains a set of specific amplification primers for SNP markers (comprising the following primers: the rs2981578 amplification primer whose sequence is SEQ ID No:1 and SEQ ID No:2 and / or whose sequence is SEQ ID No:3 and SEQ ID No:4 rs1966265, see Table 1 for details), and common reagents required by corresponding PCR techniques, such as: dNTPs, MgCl 2 , double distilled water, etc., these commonly used reagents are well known to those skilled in the art, and there may also be standards and controls (such as standards for genotype determination and blank controls, etc.). The value of this kit is that it only needs peripheral blood and no other tissue samples, detects the SNP...

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Abstract

The invention relates to an SNP marker associated with a curative effect of novel adjuvant chemotherapy of a breast cancer and an application of the SNP marker, and particularly relates to an SNP marker associated with the curative effect of novel adjuvant taxol chemotherapy of the breast cancer and an application of the SNP marker. The SNP marker comprises an rs2981578 site on an FGFR2 gene and an rs1966265 site on an FGFR4 gene. The application is the application as the SNP marker. The invention further provides a kit for detecting or predicting the curative effect of novel adjuvant chemotherapy of the breast cancer. The SNP marker can be applied to detection or prediction of the curative effect of the novel adjuvant taxol chemotherapy of the breast cancer, a basis is provided for implementation of individualized treatment of a patient, and the SNP marker is used for guiding individualized treatment, improving prognosis of the patient and improving the effectiveness and the security of clinical medication.

Description

technical field [0001] The invention relates to the technical fields of genetic engineering and oncology, in particular, the invention relates to a SNP marker related to the curative effect of neoadjuvant chemotherapy for breast cancer and its application. Background technique [0002] Breast cancer is one of the most common malignant tumors in women, accounting for 7-10% of all kinds of malignant tumors in the whole body, ranking first in the incidence of malignant tumors in women. Worldwide, 1.2 million women suffer from breast cancer every year, and 500,000 women die from breast cancer. Chemotherapy has become the main means of tumor treatment just like surgery and radiotherapy. Breast cancer neoadjuvant chemotherapy (Neoadjuvant Chemotherapy, NACT) is also called preoperative chemotherapy or initial chemotherapy. At present, many neoadjuvant chemotherapy regimens can not only be used for patients with advanced breast cancer, but also benefit more and more patients with...

Claims

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Application Information

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IPC IPC(8): C12Q1/68C12N15/11
CPCC12Q1/6886C12Q2600/106C12Q2600/156
Inventor 李群益戚慧洁陈璐叶婷钟明康施孝金
Owner AFFILIATED HUSN HOSPITAL OF FUDAN UNIV
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