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Preparation method of nisin aqueous solution with high clarity and high potency

A technology of nisin and aqueous solution, which is applied in the fields of chemical instruments and methods, food science, decapeptide, etc., can solve the problems of low titer and insufficient clarity of nisin aqueous solution, achieve high titer and reduce transportation costs , the effect of less salt content

Inactive Publication Date: 2017-10-24
ZHEJIANG SILVER ELEPHANT BIO ENG
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Problems solved by technology

[0004] The purpose of the present invention is to solve the technical problem that the titer of current nisin aqueous solution is low and not clear enough

Method used

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Embodiment Construction

[0018] The present invention is described in further detail in conjunction with embodiment now, and the application of the present invention is not limited to following embodiment, and any form modification of the present invention all will fall into protection domain of the present invention.

[0019] The preparation method of high clarity and high titer nisin aqueous solution of the present invention comprises the following steps:

[0020] (1) Take nisin powder with a potency greater than 35,000 IU / mg and put it into distilled water to prepare an aqueous solution with a potency of 50,000 IU / ml;

[0021] (2) The above aqueous solution was subjected to ultrafiltration treatment through a 2000D ultrafiltration membrane to obtain a clear aqueous solution; it should be noted that during the trial production, three specifications of ultrafiltration membranes of 3000D, 2000D and 1000D were selected and tested one by one, and it was found that the 3000D ultrafiltration membrane The ...

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Abstract

The invention discloses a preparation method of a nisin aqueous solution with high clarity and high potency. The preparation method comprises the following steps: (1), taking nisin powder of which the potency is larger than 35000 IU / mg, and adding into distilled water to prepare an aqueous solution of which the potency is 50000 IU / mg; (2), carrying out ultrafiltration treatment on the aqueous solution through a 2000 D ultrafiltration membrane, so as to obtain a clear aqueous solution; (3), filtering the clear aqueous solution through 0.2 mu m microporous filtering membrane after sterilizing for 10 min under the temperature of 80 DEG C, so as to obtain the nisin aqueous solution. The nisin aqueous solution is prepared by taking high-purity nisin as a raw material, filtering through the ultrafiltration membrane, desalting and condensing by a reverse osmose membrane, the potency of the nisin aqueous solution is higher than 1000000 IU / mL, can be placed for one year without turbidity, and can be widely used in preservation of beverages, healthcare products, green products and the like.

Description

technical field [0001] The invention relates to a method for preparing chemicals, in particular to a method for preparing an aqueous nisin solution with high clarity and high titer. Background technique [0002] Nisin, also known as nisin or nisin, is a natural and highly effective food additive that can inhibit Gram-positive bacteria and is widely used in the preservation of various foods and beverages. Nisin has been used in food preservation for more than 10 years. [0003] Most of the commercially available nisin is its compound with sodium chloride, the potency is low, only about 1000 IU / mg, the purity is about 2.5%, it is relatively turbid, and cannot be dissolved. Contents of the invention [0004] The purpose of the present invention is to solve the technical problem that the nisin aqueous solution is low in titer and not clear enough. [0005] In order to solve the above technical problems, the present invention provides a method for preparing an aqueous nisin s...

Claims

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Application Information

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IPC IPC(8): C07K14/315C07K1/34A23L2/44
CPCC07K14/315A23L2/44
Inventor 李光进金陈斌叶艇陈艺强周斌
Owner ZHEJIANG SILVER ELEPHANT BIO ENG
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