A method for rapid determination of sulfonamides and quinolones residues in eggs

Abstract

The invention discloses a method for rapidly testing drug residues of sulfonamides and quinolones in chicken eggs, and relates to the technical field of chicken egg sample detection. The method comprises the following steps: extracting a chicken egg sample with 8mL of 2% formic acid acetonitrile, further adding 0.5mL of a 0.01mol / L EDTA (Ethylene Diamine Tetraacetic Acid) buffer solution of which the pH value is 7.0, oscillating, purifying by using a QuEChERS EMR-Lipid rapid sample extraction technique, performing gradient elution by using a 0.2% formic acid solution and an acetonitrile solution as mobile phases, and performing qualitative and quantitative analysis on 30 sulfonamides and quinolones through dynamic multi-reaction monitoring of an electric spraying ion source. The method is simple and feasible in pretreatment, convenient to operate, high in precision, capable of meeting rapid and accurate detection requirements on sulfonamides and quinolones in chicken eggs, and applicable to accurate qualitative and quantitative analysis on large-scale samples.

Description

technical field [0001] The invention relates to the technical field of egg sample detection, in particular to a method for rapidly determining sulfonamide and quinolone drug residues in eggs by combining QuEChERS EMR-Lipid with LC-MS / MS. Background technique [0002] Sulfa antibiotics (sulfonamides, sAs) are a class of antibacterial drugs that are artificially synthesized with the structure of p-aminobenzenesulfonamide, and quinolones (4-quinolones) are a class of antibacterial drugs containing the basic structure of 4-quinolones. With the advantages of stable chemical properties, wide antibacterial spectrum, high efficiency, low toxicity, low price, and convenient use, it is widely used in the breeding industry to treat and prevent animal disease infections, and is increasingly valued by farmers. However, due to factors such as non-standard drug use by producers or failure to comply with the correct drug withdrawal period, sAs and FQs accumulate excessively in livestock and...

AI Technical Summary

Problems solved by technology

[0003] At present, the analysis and detection methods for sAs and FQs at home and abroad mainly include microbial detection, enzyme-linked immunosorbent assay, capillary electrophoresis (CE), high performance liquid chromatography (HPLC) and high performance liquid chromatography-tandem mass spectrometry (HPLC). -MS / MS), etc., wherein, HPLC-MS / MS method is an important method for detecting veterinary drug residues in food of animal origin. This method combines liquid phase retention time and adopts multiple reaction monitoring (MRM) mode to detect Quantitative and qualitative analysis is suitable for the detection of complex matrix samples, but the current LC-MS / MS methods for the detection of sAs and FQs are mainly concentrated in aquatic products, chicken, pork and milk, etc. There is no literature report on sulfa drugs in eggs The liquid chromatography-mass spectrometry / mass spectrometry method is a fast method that can simultaneously detect sulfonamides and quinolones in eggs and has not been reported in the literature

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