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Application of zymosan-A in preparing medicine for protecting acute radioactive bone marrow injury

A zymosan, radioactive technology, applied in the field of biomedicine, to achieve the effect of anti-tumor immune function, reducing cell apoptosis rate and reducing apoptosis rate

Inactive Publication Date: 2017-10-27
SECOND MILITARY MEDICAL UNIV OF THE PEOPLES LIBERATION ARMY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] At present, there is no literature report that zymosan plays a protective role in acute radiation-induced bone marrow injury by activating the TLR2 signaling pathway

Method used

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  • Application of zymosan-A in preparing medicine for protecting acute radioactive bone marrow injury
  • Application of zymosan-A in preparing medicine for protecting acute radioactive bone marrow injury
  • Application of zymosan-A in preparing medicine for protecting acute radioactive bone marrow injury

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] Example 1: Zymosan can reduce bone marrow injury in mice after irradiation

[0030] 1. Experimental method

[0031] C57 / BL6, 8-week-old male mice were selected for the experiment, and the mice were divided into a zymosan group and a control group. 24 hours before irradiation and 2 hours before irradiation, mice were given intraperitoneal injection of zymosan (25mg / kg / time). Since zymosan was dissolved in sterile saline, the mice in the control group were given intraperitoneal injection of the same dose of sterile saline.

[0032] The mice in the zymosan group and the control group were irradiated by a single whole body irradiation, the dose was 7.0Gy, the dose rate was 1Gy / min, and the irradiation source was 60 Co gamma rays.

[0033] The bone marrow of the mice was harvested on the 1st, 5th, 10th, 15th, and 30th day after irradiation for pathological examination. The pathological examination was HE staining, showing the bone marrow tissue structure and lesions. In ad...

Embodiment 2

[0039] Example 2: Zymosan reduces the apoptosis of AHH-1 after irradiation

[0040] 1. Experimental method:

[0041] In order to verify the effect of zymosan on apoptosis after irradiation, apoptosis detection was performed on AHH-1 after irradiation.

[0042] Apoptosis was detected by AnnexinV / PI double-staining (purchased from Invitrogen) flow cytometry method, the principle of which is: apoptosis is a process of programmed initiation, which can be divided into two processes: early apoptosis and late apoptosis. During early apoptosis, phosphatidylserine (PS) can change from the original state inside the cell phospholipid membrane to outward. At this time, Annexin V can combine with the everted PS as a detection mark of early apoptosis. In the late stage of apoptosis, the permeability of the cell membrane increases. At this time, propidium iodide (PI), as a nucleic acid dye, can enter the interior of the cell and bind to the nucleic acid, and the nucleus is stained red. Ann...

Embodiment 3

[0046] Example 3: Zymosan reduces the proliferation inhibitory effect of irradiation on HIEC

[0047] 1. Experimental method:

[0048] The detection of cell proliferation ability adopts CCK-8 experiment, the principle is: WST-8 in CCK-8 reagent can be reduced to highly water-soluble yellow formazan product by dehydrogenase in cell mitochondria, and the formed formazan product The number is proportional to the number of living cells. The light absorption value was measured at a wavelength of 450nm by an enzyme-linked immunosorbent assay instrument, which can indirectly reflect the number of living cells.

[0049] The specific operation steps are as follows: 24 hours before irradiation, plate HIEC cells on a 96-well plate (5000 cells / well). / ml) and NS. The irradiation dose was 8.0Gy, and the dose rate was 1Gy / min. After the irradiation, the culture medium was replaced and the culture was continued. The light absorption value of the cells was measured with an enzyme-linked im...

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Abstract

The invention relates to the field of biomedicines, in particular to application of zymosan-A or derivative thereof in preparing a medicine for protecting acute radioactive bone marrow injury. The application is characterized in that after proofed by mouse in-vivo experiment, the zymosan-A can obviously reduce the injury degree to a bone marrow hematopoietic system due to ionizing radiation, promote the restoration of the hematopoietic system, and inhibit the decreasing of peripheral blood leucocyte caused by the ionizing radiation; after proofed by in-vitro cytology experiments, the zymosan-A can reduce the cell withering rate after radiation, and improve the cell proliferation ability after radiation; after proofed by TLR2 knocking the mouse, the zymosan-A can play the radiation protecting function after activating a TLR2 signal channel in a targeting way; after proofed by the experiments, the zymosan-A can decrease the radioactive injury of the bone marrow hematopoietic system of the mouse via activating the TLR2 signal channel, and protect the peripheral blood leucocyte, so that a new reference is provided for the zymosan-A protecting the acute radioactive bone marrow injury.

Description

technical field [0001] The invention relates to the field of biomedicine, specifically, the application of zymosan or its derivatives in the preparation of medicines for protecting acute radiation bone marrow injury, and the pharmaceutical composition containing zymosan or its derivatives. Background technique [0002] At present, the nuclear industry, nuclear energy and nuclear radiation technology have been widely used in the national economy, military and medical fields. The wide application of nuclear and radiation has greatly increased the chances of radiation workers and the public being injured by nuclear radiation. Nuclear radiation damage and protection It has also attracted more and more attention from academia and the public. After receiving large doses of ionizing radiation, exposed personnel may develop acute radiation sickness, among which acute radiation-induced bone marrow injury (also known as bone marrow-type acute radiation sickness) has attracted increasi...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K31/736A61P7/00A61P7/04A61P7/06
CPCA61K31/736
Inventor 杜继聪蔡建明高福刘聪程赢杨彦勇刘虎张沛赵海男东肃河韩家琦
Owner SECOND MILITARY MEDICAL UNIV OF THE PEOPLES LIBERATION ARMY
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