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A method for storing and transporting stem cells under normal temperature conditions and the matrix used therein

A transport method and stem cell technology, applied in the field of cell biology, can solve the problems of maintenance time period, low cell viability, death, etc., and achieve the effect of protecting weight loss and intestinal damage

Active Publication Date: 2021-03-02
UNIVERSITY OF MACAU
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] 1. The amount of cells transported is limited
[0005] 2. It is easy to cause the activity of cells to decrease and gradually die
[0006] 3. Consumes a large amount of expensive medium, for example, it takes about 35ml of medium to fill a 25ml culture bottle
Therefore, this method is cumbersome, time-consuming, and the cell viability is low, and the maintenance period

Method used

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  • A method for storing and transporting stem cells under normal temperature conditions and the matrix used therein
  • A method for storing and transporting stem cells under normal temperature conditions and the matrix used therein
  • A method for storing and transporting stem cells under normal temperature conditions and the matrix used therein

Examples

Experimental program
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Effect test

Embodiment 1

[0044] The present invention provides a stem cell preservation and transportation method under normal temperature conditions, which is characterized in that the method includes the following four steps; Step 1, the step of forming stem cell clumps, preparing a suspension culture medium, under suspension culture conditions, Spontaneous aggregation of stem cells to form clumps; step 2, step of culturing stem cell clumps, preparing an active energy liquid culture medium, placing the stem cell clumps formed in step 1 in the active energy liquid culture medium to form a stem cell clump product; step Three, the step of encapsulating and transporting the stem cell aggregate product, packaging the stem cell aggregate product in a plastic container under normal temperature conditions to achieve high-density and large-scale transportation; step four, the stem cell aggregate matrix removal step, after the cells are transported to the destination Finally, the substrate is removed by digest...

Embodiment 2

[0070] This example will demonstrate that mesenchymal stem cell spheroids still have curative effect on colitis mice after being stored at room temperature for 7 days by applying the method of the present invention.

[0071] After the mesenchymal stem cell spheres in Example 1 were stored at room temperature for 7 days, experiments were performed on 6-8 week-old mice. Experiments were performed on female C57BL / 6 mice using dextran sulfate sodium (DSS)-induced colitis model. The experimental steps are as follows:

[0072] 1. Mix 2% (w / v) DSS (molecular weight 36,000-50,000, MP Biomedical) in the drinking water for 7 days and let the mice drink it.

[0073] 2. Resuspend the mesenchymal stem cell spheres in PBS to obtain a concentration equivalent to 1×106 cells / 100 microliters.

[0074] 3. On days 1 and 2 after the start of DSS treatment, use a syringe with a 21-gauge needle to intraperitoneally (i.p.) inject 100 μl of MSC sphere suspension (without sphere dissociation) or PB...

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Abstract

The invention provides a method for storing and transporting stem cells at normal temperature, and provides an energy matrix for ensuring their survival rate and biological activity. The method includes agglomerating stem cells using their own characteristics under three-dimensional suspension conditions to naturally reduce their proliferation and metabolic rate, and can guarantee their survival rate and biological activity for up to 7-10 days under normal temperature conditions. The method of the present invention is simple and reliable, low in price, stable and efficient, and high in safety, and can prepare, store, transport and use stem cells on a large scale under normal temperature conditions, without any temperature maintenance equipment, screening and purification steps, and can play a role in the organization of stem cells Biological functions such as regeneration and immune regulation are directly used in stem cell biology and medical science research, immune diseases and other application requirements.

Description

technical field [0001] The invention relates to the field of cell biology, in particular to a method for preserving, transporting and using cells capable of aggregating into clusters under normal temperature conditions, and ensuring their survival rate and biological activity. Background technique [0002] Cells are the basic unit of life, and the human body is composed of more than 200 types of cells, such as cardiomyocytes, blood cells and stem cells. Stem cells are cells that have the ability to self-renew and differentiate. For example, mesenchymal stem cells are a type of cells derived from the mesoderm, which are widely found in adult and fetal tissues such as bone marrow, fat, connective tissue, umbilical cord and placenta. Mesenchymal stem cells have the function of self-renewal and can differentiate into bone, cartilage, muscle, fat, and even nerve and liver cells under specific conditions. In addition, mesenchymal stem cells have low immunogenicity and can regula...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01N1/02
CPCA01N1/02A01N1/0231
Inventor 徐仁和蒋斌燕丽
Owner UNIVERSITY OF MACAU
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