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Method for preserving and transporting stem cells under ambient temperature condition, and substrate used therein

A transportation method and stem cell technology, applied in the field of cell biology, can solve problems such as maintenance time period, low cell viability, death, etc., and achieve the effect of protecting weight loss and intestinal damage

Active Publication Date: 2017-11-03
UNIVERSITY OF MACAU
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] 1. The amount of cells transported is limited
[0005] 2. It is easy to cause the activity of cells to decrease and gradually die
[0006] 3. Consumes a large amount of expensive medium, for example, it takes about 35ml of medium to fill a 25ml culture bottle
Therefore, this method is cumbersome, time-consuming, and the cell viability is low, and the maintenance period

Method used

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  • Method for preserving and transporting stem cells under ambient temperature condition, and substrate used therein
  • Method for preserving and transporting stem cells under ambient temperature condition, and substrate used therein
  • Method for preserving and transporting stem cells under ambient temperature condition, and substrate used therein

Examples

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Effect test

Embodiment 1

[0044] The present invention provides a stem cell preservation and transportation method under normal temperature conditions, which is characterized in that the method includes the following four steps; Step 1, the step of forming stem cell clumps, preparing a suspension culture medium, under suspension culture conditions, Spontaneous aggregation of stem cells to form clumps; step 2, step of culturing stem cell clumps, preparing an active energy liquid culture medium, placing the stem cell clumps formed in step 1 in the active energy liquid culture medium to form a stem cell clump product; step Three, the step of encapsulating and transporting the stem cell aggregate product, packaging the stem cell aggregate product in a plastic container under normal temperature conditions to achieve high-density and large-scale transportation; step four, the stem cell aggregate matrix removal step, after the cells are transported to the destination Finally, the substrate is removed by digest...

Embodiment 2

[0070] This example will demonstrate that mesenchymal stem cell spheroids still have curative effect on colitis mice after being stored at room temperature for 7 days by applying the method of the present invention.

[0071] After the mesenchymal stem cell spheres in Example 1 were stored at room temperature for 7 days, experiments were performed on 6-8 week-old mice. Experiments were performed on female C57BL / 6 mice using dextran sulfate sodium (DSS)-induced colitis model. The experimental steps are as follows:

[0072] 1. Mix 2% (w / v) DSS (molecular weight 36,000-50,000, MP Biomedical) in the drinking water for 7 days and let the mice drink it.

[0073] 2. Resuspend the mesenchymal stem cell spheres in PBS to obtain a concentration equivalent to 1×106 cells / 100 microliters.

[0074] 3. On days 1 and 2 after the start of DSS treatment, use a syringe with a 21-gauge needle to intraperitoneally (i.p.) inject 100 μl of MSC sphere suspension (without sphere dissociation) or PB...

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Abstract

The invention provides a method for preserving and transporting stem cells under an ambient temperature condition, and an energy substrate used for maintaining the survival rate and biological activity of the stem cells. According to the method, stem cells are agglomerated under a three-dimensional suspension condition in virtue of the characteristics of the stem cells, so the propagation and metabolism rates of the stem cells naturally decrease, and the survival rate and biological activity of the stem cells can be maintained in a period of as long as 7 to 10 d under the ambient temperature condition. The method is simple, reliable, stable, highly efficient, low in cost and high in security; the method allows stem cells to be prepared, stored, transported and used in large quantities under the ambient temperature condition; the biological functions of tissue regeneration, immunoloregulation and the like of stem cells can be given to play without any temperature maintenance equipment or screening and purifying steps; and the method can directly meet application demands of biological and medical research on stem cells, cell therapy of immune diseases, etc.

Description

technical field [0001] The invention relates to the field of cell biology, in particular to a method for preserving, transporting and using cells capable of aggregating into clusters under normal temperature conditions, and ensuring their survival rate and biological activity. Background technique [0002] Cells are the basic unit of life, and the human body is composed of more than 200 types of cells, such as cardiomyocytes, blood cells and stem cells. Stem cells are cells that have the ability to self-renew and differentiate. For example, mesenchymal stem cells are a type of cells derived from the mesoderm, which are widely found in adult and fetal tissues such as bone marrow, fat, connective tissue, umbilical cord and placenta. Mesenchymal stem cells have the function of self-renewal and can differentiate into bone, cartilage, muscle, fat, and even nerve and liver cells under specific conditions. In addition, mesenchymal stem cells have low immunogenicity and can regula...

Claims

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Application Information

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IPC IPC(8): A01N1/02
CPCA01N1/02A01N1/0231
Inventor 徐仁和蒋斌燕丽
Owner UNIVERSITY OF MACAU
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