Attenuated live vaccine used for preventing toxoplasma infection and application of attenuated live vaccine

A technology of live attenuated vaccines and Toxoplasma gondii, which is applied in anti-infective drugs, protozoan antigen components, drug combinations, etc., can solve the problem of limitation, achieve huge application value, strong immune protection, and prevent normal infection

Inactive Publication Date: 2017-11-03
LANZHOU INST OF VETERINARY SCI CHINESE ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the only live attenuated Toxoplasma gondii vaccine that can be marketed in the world is S48, but it is limited to the application of local goats and sheep

Method used

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  • Attenuated live vaccine used for preventing toxoplasma infection and application of attenuated live vaccine
  • Attenuated live vaccine used for preventing toxoplasma infection and application of attenuated live vaccine
  • Attenuated live vaccine used for preventing toxoplasma infection and application of attenuated live vaccine

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Embodiment Construction

[0018] The present invention will be further described below with reference to the accompanying drawings. It should be noted that the following examples are based on the technical solution, and provide detailed implementations and specific operation processes, but the scope of the present invention is not limited to this implementation. example.

[0019] 1. Preparation of live attenuated vaccines

[0020] Inoculate HFF cells into a 25T cell culture flask, add 8 ml of 10% FBS in DMEM medium, and at 37 °C CO 2 Cultivated in an incubator. After the cells are completely confluent, change to 8ml of DMEM medium with 2% FBS and add 100ul of RHΔGRA17 strains at 37°C CO. 2 Culture in an incubator until Toxoplasma gondii escapes from the cells, collect the RHΔGRA17 tachyzoites that have just escaped from the HFF, pass the cell suspension through a 3 μm filter to remove cell debris, and then adjust the concentration of tachyzoites to 500 with sterile PBS / 200μL.

[0021] The immunizat...

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Abstract

The invention discloses an attenuated live vaccine used for preventing toxoplasma infection and application of the attenuated live vaccine. The attenuated live vaccine takes PBS as a solvent to prepare a tachyzoite suspension of a toxoplasmosis attenuative strain RHdetltaGRA17. An immune preparation for the attenuated live vaccine is established, the immunization procedure and the inoculum size of the vaccine are explored, and the animal experiment shows that the attenuated live vaccine has a stronger immunoprotection function for the acute infection, the chronic infection and the congenital infection of toxoplasmosis, the normal infection of the toxoplasmosis is prevented, meanwhile, the mother-to-fetus vertical propagation of the toxoplasmosis is effectively stopped, and the vaccine has a huge application value, and can be used for preventing the acute infection, the chronic infection and the congenital infection of toxoplasmosis.

Description

technical field [0001] The invention relates to the field of live attenuated vaccines of Toxoplasma gondii, in particular to a live attenuated vaccine for preventing Toxoplasma gondii infection and its application. Background technique [0002] Toxoplasmosis is a zoonotic parasitic disease caused by Toxoplasma gondii. About one-third of the world's population is infected. Infection of Toxoplasma gondii during pregnancy, Toxoplasma gondii can be transmitted vertically to the fetus through the placenta and then cause pregnancy miscarriage, premature birth, fetal malformation, stillbirth and hinder the normal growth of young children, which seriously endangers the reproductive health of human beings. Toxoplasma gondii can infect almost all warm-blooded animals, is highly infectious, has a wide range of epidemics, and has a wide host spectrum. The disease can still be controlled with drugs in the acute phase, but once it turns to the chronic phase, there is currently no cure. ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K39/002A61P33/02
CPCA61K39/002
Inventor 王金磊黄思扬贺君君李法财朱伟宁陈凯朱兴全
Owner LANZHOU INST OF VETERINARY SCI CHINESE ACAD OF AGRI SCI
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