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Yeast strain for highly yielding cAMP, and application thereof

A yeast, yield technology, applied in the fields of genetic engineering and microbial fermentation

Active Publication Date: 2017-11-03
TIANJIN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

It is a pity that the current research at home and abroad is focused on the signal regulation function of intracellular cAMP, and there is no special research that focuses on the extracellular production of cAMP as a product

Method used

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  • Yeast strain for highly yielding cAMP, and application thereof
  • Yeast strain for highly yielding cAMP, and application thereof
  • Yeast strain for highly yielding cAMP, and application thereof

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Experimental program
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Embodiment Construction

[0039] The invention provides a genetically modified yeast strain capable of excessively synthesizing extracellular cAMP, a recombinant method and application for constructing the strain. More specifically,

[0040] As described above, the yeast of the present invention includes the first genetic modification and the second genetic modification. Further, the yeast also includes the third, fourth and / or fifth genetic modification. Among them, the first genetic modification eliminates the feedback inhibition of cAMP, but at the same time inhibits the growth of yeast; the second genetic modification eliminates the growth inhibition caused by the first genetic modification, so that the yeast can grow normally; the third Genetic modification reduces the degradation of cAMP, and the fourth genetic modification increases the positive regulation of the synthesis of cAMP precursors in the purine synthesis pathway, which increases the synthesis of cAMP precursors and increases the prod...

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Abstract

The invention provides a yeast strain capable of excessively synthesizing extracellular cAMP, a constructing method and a fermentation technology thereof, and an application of the yeast strain in the fields of medicines, livestock husbandry, foods, health foods or chemical industry. The yeast strain comprises first gene modification and second gene modification, wherein the first genes are protein kinase A (PKA) catalytic subunit coding genes TPK1, TPK2 and TPK3, the first genes are modified to completely inhibit the activity or expression of the PKA in order to eliminate the feedback inhibition of cyclic adenosine monophosphate (cAMP) and inhibit the growth of yeast, and the second gene modification eliminates the first gene modification induced growth inhibition in order to make yeast grow normally and increase the cAMP yield of the yeast. The yeast strain further includes third gene modification, fourth gene modification, and / or fifth gene modification. The recombinant yeast strain can stably, continuously and highly-efficiently produce the extracellular cAMP.

Description

technical field [0001] The invention belongs to the technical field of genetic engineering and microbial fermentation, and specifically relates to a yeast strain capable of excessively synthesizing extracellular cAMP, its construction method, fermentation process and application in the fields of medicine, animal husbandry, food, health products or chemical industry. Background technique [0002] Cyclic adenosine monophosphate (cAMP) is an important physiologically active substance that widely exists in the human body. play an important regulatory role. At present, cAMP is widely used in medicine: clinically, cAMP is used to treat angina pectoris, myocardial infarction, myocarditis and cardiogenic shock, etc.; Combined with the curative effect of chemotherapy, it can also be used to induce remission of acute leukemia; in addition, it also has certain curative effect on elderly chronic bronchitis, various hepatitis and psoriasis. cAMP can also be used as a drug intermediate ...

Claims

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Application Information

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IPC IPC(8): C12N1/19C12P19/32A23K10/18A23K20/163C12R1/865C12R1/84C12R1/72
CPCA23K10/18A23K20/163C12N9/1205C12N9/16C12P19/32C12Y301/04053
Inventor 邹少兰高文萱胡芸杨顺华马平生
Owner TIANJIN UNIV
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