Preservative based on enzymatic hydrolysate of fish skin and preparation method of preservative
The technology of a preservative and an enzymatic hydrolysate is applied in the field of a preservative based on the enzymatic hydrolysate of fish skin and its preparation field, which can solve the problems such as the need to improve the antioxidant capacity, the narrow antibacterial range of the preservative, the influence on food safety, and the like, and achieve product quality. Good quality stability, good economy, good fresh-keeping effect
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Embodiment 1
[0015] The antistaling agent based on fish skin enzymatic hydrolyzate, the ingredients and parts by weight of the antistaling agent are: 5 parts of fish skin enzymatic hydrolyzate, 2 parts of pectin enzymatic hydrolyzate, and its preparation comprises the following steps:
[0016] 1) Preparation of enzymatic hydrolyzate of fish skin: Mix 5 parts of fish skin with 10 parts of water, add phosphatase in an amount of 40IU / g, enzymolyze at pH 8 and temperature 30°C for 30min, and end the enzymolysis Afterwards, inactivate the enzyme activity at a temperature of 85° C. for 10 minutes, and filter. Mix the filtered fish skin with 5 parts of water, add pepsin in an amount of 50IU / g, and carry out enzymatic hydrolysis for 2 hours at a pH of 2 and a temperature of 90°C, and continue at a temperature of 95°C after the enzymatic hydrolysis Inactivate the enzyme for 15 minutes, filter through a ceramic membrane with a pore size of 70 nm, and take the supernatant to obtain fish skin enzymati...
Embodiment 2
[0020] The antistaling agent based on fish skin hydrolyzate, the composition and parts by weight of the antistaling agent are: 6 parts of fish skin hydrolyzate, 3 parts of pectin hydrolyzate, its preparation comprises the following steps: (1) fish skin Preparation of enzymatic hydrolyzate: Mix 6 parts of fish skin with 12 parts of water, add lipase, the addition amount is 50IU / g, enzymolyze at pH 8, temperature 32°C for 32min, after the enzymolysis is completed, the temperature is 85 Continue to inactivate the enzyme activity at ℃ for 10 minutes, and filter. Mix the filtered fish skin with 6 parts of water, add protease in an amount of 60IU / g, and carry out enzymatic hydrolysis for 2.5 hours under the conditions of pH 2 and temperature 92°C, and continue at a temperature of 95°C after enzymolysis Inactivate the enzyme for 15 minutes, filter through a ceramic membrane with a pore size of 90 nm, and take the supernatant to obtain the fish skin enzymatic hydrolyzate. The content...
Embodiment 3
[0022] The antistaling agent based on fish skin enzymatic hydrolyzate, the ingredients and parts by weight of the antistaling agent are: 10 parts of fish skin enzymatic hydrolyzate, 8 parts of pectin enzymatic hydrolyzate, and its preparation comprises the following steps:
[0023] 1) Preparation of enzymatic hydrolyzate of fish skin: Mix 10 parts of fish skin with 15 parts of water, add lipase in an amount of 80IU / g, enzymatically hydrolyze for 40min at pH 9 and temperature 35°C, and end the enzymatic hydrolysis Afterwards, inactivate the enzyme activity at a temperature of 85° C. for 10 minutes, and filter. Mix the filtered fish skin with 10 parts of water, add protease in an amount of 80IU / g, under the conditions of pH 3 and temperature 100°C, carry out enzymatic hydrolysis for 3 hours, and after the end of the enzymatic hydrolysis, continue for 15 minutes at a temperature of 95°C Inactivate the enzyme activity, filter with a ceramic membrane with a pore size of 180 nm, and...
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