A method for assisted selection of pericarp anthocyanin-synthesized fresh corn inbred lines based on ligule color

A technology of fresh corn and inbred lines, applied in the field of crop genetics and breeding, can solve the problems of long breeding cycle of seed color traits, etc., achieve the effects of reducing workload, speeding up the breeding process, and improving breeding efficiency

Inactive Publication Date: 2019-06-07
INST OF TOBACCO ANHUI ACADEMY OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The purpose of the present invention is to overcome the deficiencies of the prior art and provide a method for assisting the breeding of pericarp anthocyanin-synthetic fresh corn inbred lines based on ligule color to solve the problem of directional improvement and cultivation of pericarp anthocyanin-synthetic fresh corn. When eating corn inbred lines, the technical problem of long breeding cycle is caused by the fact that the F1 and F2 generations do not segregate the color traits of the seeds

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] The present invention provides a method for assisting in the breeding of pericarp anthocyanin-synthesized fresh corn inbred lines by using ligule color traits, comprising the following steps:

[0021] (1) cross the corn inbred line 0414 with black pericarp and the corn inbred line 3370 with colorless pericarp to obtain the F1 generation;

[0022] (2) The F1 generation is self-bred and reserved to obtain the F2 generation;

[0023] (3) Sowing the F2 generation in a mixed manner, selecting a single plant whose ligule color is purple, and taking into account the selection of a single plant with good phenotypic traits such as plant type, leaf type, and disease resistance, to carry out selfing and retain a single ear to obtain the F3 generation;

[0024] (4) Plant the F3 generation by panicle row, select a single plant with purple ligule color in the panicle row, and select a single plant with good phenotypic traits such as plant type, leaf type, and disease resistance to ca...

Embodiment 2

[0030] The present invention provides another method for assisting the breeding of pericarp anthocyanin-synthesized fresh corn inbred lines by using the ligule color trait, comprising the following steps:

[0031] (1) The maize inbred line 0425 with purple pericarp was crossed with the maize inbred line 3370 with colorless pericarp to obtain the F1 generation;

[0032] (2) The F1 generation is self-bred and reserved to obtain the F2 generation;

[0033] (3) Sowing the F2 generation in a mixed manner, selecting a single plant whose ligule color is purple, and taking into account the selection of a single plant with good phenotypic traits such as plant type, leaf type, and disease resistance, to carry out selfing and retain a single ear to obtain the F3 generation;

[0034] (4) Plant the F3 generation by panicle row, select a single plant with purple ligule color in the panicle row, and select a single plant with good phenotypic traits such as plant type, leaf type, and disease ...

Embodiment 3

[0039] The present invention provides another method for assisting the breeding of pericarp anthocyanin-synthesized fresh corn inbred lines by using the ligule color trait, comprising the following steps:

[0040] (1) cross the corn inbred line 0414 with black pericarp and the corn inbred line 3370 with colorless pericarp to obtain the F1 generation;

[0041] (2) The F1 generation is self-bred and reserved to obtain the F2 generation;

[0042] (3) Sowing the F2 generation in a mixed manner, selecting a single plant whose ligule color is purple, and taking into account the selection of a single plant with good phenotypic traits such as plant type, leaf type, and disease resistance, to carry out selfing and retain a single ear to obtain the F3 generation;

[0043] (4) Plant the F3 generation by panicle row, select a single plant with purple ligule color in the panicle row, and select a single plant with good phenotypic traits such as plant type, leaf type, and disease resistance...

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Abstract

The invention discloses a method for assisting the breeding of pericarp anthocyanin-synthesized fresh corn inbred lines based on ligule color. The method is in the breeding process of pericarp anthocyanin-synthesized fresh corn inbred lines Among them, according to the color traits of the ligule, the pericarp anthocyanin-synthesized fresh corn inbred line was selected. Hybrid selection, F1 generation self-bred seedlings, in the F2 generation, according to the color traits of the ligule, select individual plants with purple ligules for self-bred seedlings, plant according to panicle rows, and select individual plants with good phenotype traits , several generations of continuous selfing selection, and stable hereditary pericarp anthocyanin synthetic maize inbred lines can be obtained. Compared with the prior art, the method can speed up the breeding process of the pericarp anthocyanin-synthesized fresh corn inbred line, reduce the workload, and improve the breeding efficiency of the colored corn varieties.

Description

technical field [0001] The invention relates to the technical field of crop genetics and breeding, in particular to a method for assisting in the breeding of pericarp anthocyanin-synthesized fresh corn inbred lines using the ligule color trait. Background technique [0002] Anthocyanin is a water-soluble natural pigment synthesized in plants. Anthocyanin is a powerful antioxidant that can bring many benefits to the human body, such as protecting the human body from free radical damage, strengthening blood vessels Elasticity, improved circulation and more. Anthocyanins have been found in a variety of food crops, such as corn, rice, sorghum, purple sweet potato and so on. [0003] Fresh purple, black and other colorful corns are rich in anthocyanins, because of their high anthocyanins content, fat, protein, iron, calcium, amino acids and other nutrients, they are considered as health-care golden food in fresh corns . Peel rich in anthocyanins is a kind of colorful fresh cor...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H1/04A01H1/02
CPCA01H1/02A01H1/04
Inventor 李廷春杨华应董庆周应兵陈洪俭
Owner INST OF TOBACCO ANHUI ACADEMY OF AGRI SCI
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