A method for assisted selection of pericarp anthocyanin-synthesized fresh corn inbred lines based on ligule color
A technology of fresh corn and inbred lines, applied in the field of crop genetics and breeding, can solve the problems of long breeding cycle of seed color traits, etc., achieve the effects of reducing workload, speeding up the breeding process, and improving breeding efficiency
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Embodiment 1
[0020] The present invention provides a method for assisting in the breeding of pericarp anthocyanin-synthesized fresh corn inbred lines by using ligule color traits, comprising the following steps:
[0021] (1) cross the corn inbred line 0414 with black pericarp and the corn inbred line 3370 with colorless pericarp to obtain the F1 generation;
[0022] (2) The F1 generation is self-bred and reserved to obtain the F2 generation;
[0023] (3) Sowing the F2 generation in a mixed manner, selecting a single plant whose ligule color is purple, and taking into account the selection of a single plant with good phenotypic traits such as plant type, leaf type, and disease resistance, to carry out selfing and retain a single ear to obtain the F3 generation;
[0024] (4) Plant the F3 generation by panicle row, select a single plant with purple ligule color in the panicle row, and select a single plant with good phenotypic traits such as plant type, leaf type, and disease resistance to ca...
Embodiment 2
[0030] The present invention provides another method for assisting the breeding of pericarp anthocyanin-synthesized fresh corn inbred lines by using the ligule color trait, comprising the following steps:
[0031] (1) The maize inbred line 0425 with purple pericarp was crossed with the maize inbred line 3370 with colorless pericarp to obtain the F1 generation;
[0032] (2) The F1 generation is self-bred and reserved to obtain the F2 generation;
[0033] (3) Sowing the F2 generation in a mixed manner, selecting a single plant whose ligule color is purple, and taking into account the selection of a single plant with good phenotypic traits such as plant type, leaf type, and disease resistance, to carry out selfing and retain a single ear to obtain the F3 generation;
[0034] (4) Plant the F3 generation by panicle row, select a single plant with purple ligule color in the panicle row, and select a single plant with good phenotypic traits such as plant type, leaf type, and disease ...
Embodiment 3
[0039] The present invention provides another method for assisting the breeding of pericarp anthocyanin-synthesized fresh corn inbred lines by using the ligule color trait, comprising the following steps:
[0040] (1) cross the corn inbred line 0414 with black pericarp and the corn inbred line 3370 with colorless pericarp to obtain the F1 generation;
[0041] (2) The F1 generation is self-bred and reserved to obtain the F2 generation;
[0042] (3) Sowing the F2 generation in a mixed manner, selecting a single plant whose ligule color is purple, and taking into account the selection of a single plant with good phenotypic traits such as plant type, leaf type, and disease resistance, to carry out selfing and retain a single ear to obtain the F3 generation;
[0043] (4) Plant the F3 generation by panicle row, select a single plant with purple ligule color in the panicle row, and select a single plant with good phenotypic traits such as plant type, leaf type, and disease resistance...
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